Oct 3/4 antibody | MyBioSource MBS535995 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Oct 3/4 antibody

catalog :

MBS535995

quantity :

0.1 mg

price :

340 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS535995

products type :

Antibody

products full name :

Oct 3/4 antibody

products short name :

Oct 3/4

products name syn :

Polyclonal Oct 3/4; Anti-Oct 3/4; Octamer-Binding Transcription Factor 3/4

other names :

octamer-binding transcription factor 3, partial; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 1; NF-A3; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3

products gene name :

Oct 3/4

other gene names :

Pou5f1; Pou5f1; Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7; Oct-3; Oct-4; Otf-3; Otf3; Oct-3; Oct-4; OTF-3

uniprot entry name :

PO5F1_MOUSE

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

179

purity :

Oct 3/4 antibody was purified by affinity chromatography.

form :

Affinity purified and supplied in PBS, with 0.02% NaN3.

concentration :

1.0 mg/ml

storage stability :

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

tested application :

Immunohistochemistry (IHC), Western Blot (WB)

app notes :

IHC: 1:100-1:250. WB: 1:500-1:1,000

other info1 :

Biological Significance: Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm.

other info2 :

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Oct 3/4 antibody was raised in rabbit using an internal sequence of the human Oct 3/4 protein as the immunogen.

products categories :

Differentiation & Development ; Purified Polyclonal Antibodies

products description :

Affinity purified Rabbit polyclonal Oct 3/4 antibody

ncbi gi num :

212720399

ncbi acc num :

BAG84139.1

uniprot acc num :

P20263

ncbi mol weight :

38,216 Da

ncbi pathways :

Developmental Biology Pathway (1167944); PluriNetWork Pathway (198307); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026143); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1167995); Wnt Signaling Pathway And Pluripotency (198412)

uniprot summary :

Oct4: Transcription factor that binds to the octamer motif (5 -ATTTGCAT-3 ). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytoplasm; nucleolus; nucleus; cytosol. Molecular Function: protein binding; miRNA binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; chromatin binding; transcription corepressor activity; transcription factor activity; transcription factor binding. Biological Process: transcription from RNA polymerase II promoter; negative regulation of calcium ion-dependent exocytosis; endodermal cell fate commitment; somatic stem cell maintenance; multicellular organismal development; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; ectodermal cell fate commitment; negative regulation of transcription from RNA polymerase II promoter; mesodermal cell fate commitment; response to organic substance; regulation of transcription, DNA-dependent; blastocyst growth; cell fate commitment; response to retinoic acid; negative regulation of protein kinase B signaling cascade; transcription, DNA-dependent; stem cell maintenance; germ-line stem cell maintenance; stem cell differentiation; mRNA transcription from RNA polymerase II promoter; trophectodermal cell differentiation; positive regulation of protein kinase B signaling cascade; negative regulation of cell differentiation; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

size1 :

0.1 mg

price1 :

340 USD

more info or order :

MyBioSource product webpage