antibody

Plectin antibody

MBS534396

0.1 ml

655 USD

polyclonal

guinea pigs

nonconjugated

human, mouse, cow

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Plectin antibody

Plectin

Polyclonal Plectin; Anti-Plectin

plectin; Plectin; plectin; plectin; Hemidesmosomal protein 1; HD1; Plectin-1

PLEC; PLEC; HD1; PCN; EBS1; EBSO; PLTN; PLEC1; LGMD2Q; PLEC1b; PLEC1; PCN; PLTN; HD1

PLEC_HUMAN

Polyclonal

Guinea Pig

Bovine, Fish, Frog, Human, Mouse

4684

Human plectin

Whole antiserum

Supplied as whole antiserum with 0.09% NaN3.

Store at 4 degree C.

Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)

IHC-F:1:500. IHC-P: 1:500. WB: 1:16,000

Biological Significance: Plectin is a giant protein (c500 kDa) found in nearly all mammalian cells which acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. In addition plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells.

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Plectin antibody was raised in guinea pig using the C-Terminal "C" domain of recombinant human plectin as the immunogen.

Cell Biology

Guinea Pig polyclonal Plectin antibody

1296662

CAA91196.1

Q15149

514,775 Da

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway 730306!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 105679!!Cell Junction Organization Pathway 160966!!Cell-Cell Communication Pathway 477132!!Collagen Formation Pathway 645288!!EGFR1 Signaling Pathway 198782

Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011]

0.1 ml

655 USD