Collagen Type I antibody (biotin) | MyBioSource MBS534202 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Collagen Type I antibody (biotin)

catalog :

MBS534202

quantity :

0.1 mg

price :

695 USD

clonality :

polyclonal

host :

rabbit

conjugate :

biotin

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.

image 2 :

Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Collagen Type I antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

image 3 :

FACS analysis of mouse lung cells. Collagen type I antibody (biotin) detected with PE-conjugated CD45 and PE-conjugated collagen type I secondary antibody.

product information

catalog number :

MBS534202

products type :

Antibody

products full name :

Collagen Type I antibody (biotin)

products short name :

[Collagen Type I]

products name syn :

[Polyclonal Collagen Type I; Anti-Collagen Type I; OI4; Collagen type I alpha 2; Osteogenesis Imperfecta Type IV; Collagen type I alpha 1; Collagen Of Skin Tendon And Bone; Collagen Type 1]

other names :

[collagen, type I, alpha 1, isoform CRA_a; Collagen alpha-1(I) chain; collagen alpha-1(I) chain; collagen, type I, alpha 1; Alpha-1 type I collagen]

other gene names :

[COL1A1; COL1A1; OI1; OI2; OI3; OI4; EDSC]

uniprot entry name :

CO1A1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens.

sequence length :

885

specificity :

Bovine/Human collagen type I

purity :

Collagen type I antibody (biotin) was purified by affinity chromatography.

form :

Supplied a lyophilized from 20mM Potassium Phosphate, 150mM Sodium Chloride, pH 7.2, with 10 mg/ml BSA and 0.01% Sodium Azide as a preservative. Reconstitute with 0.1ml of deionizd water.

concentration :

1.0 mg/ml (by UV absorbance at 280 nm) after reconstitution.

storage stability :

Store at 4°C prior to reconstitution. Stable at 4°C as an undiluted liquid. For longer storage mix with an equal volume of glycerol and store at -20°C.

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

app notes :

IP: 1:100. IHC: 1:50-1:200. ELISA: 1:3,000-1:6,000. WB: 1:3,000-1:6,000

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Immunogen: Collagen Type I from human and bovine placenta. Bioactivity: Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

other info2 :

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

products categories :

Cell Biology ; Conjugated Polyclonal Antibodies

products description :

Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-I collagen is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone.

ncbi gi num :

119615037

ncbi acc num :

EAW94631.1

uniprot acc num :

P02452

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795)

ncbi summary :

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

uniprot summary :

COL1A1: Type I collagen is a member of group I collagen (fibrillar forming collagen). Defects in COL1A1 are the cause of Caffey disease (CAFFD); also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A); also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1). A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2); also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3). A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4); also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP); also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture. A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. Belongs to the fibrillar collagen family. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 17q21.33. Cellular Component: Golgi apparatus; extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type I; extracellular region; secretory granule. Molecular Function: identical protein binding; protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent. Biological Process: response to peptide hormone stimulus; intramembranous ossification; extracellular matrix organization and biogenesis; response to cAMP; collagen fibril organization; embryonic skeletal development; positive regulation of transcription, DNA-dependent; response to estradiol stimulus; response to corticosteroid stimulus; extracellular matrix disassembly; protein transport; sensory perception of sound; visual perception; collagen biosynthetic process; skeletal development; endochondral ossification; response to drug; platelet activation; receptor-mediated endocytosis; blood vessel development; skin morphogenesis; osteoblast differentiation; collagen catabolic process; response to hyperoxia; response to hydrogen peroxide; blood coagulation; leukocyte migration; positive regulation of cell migration. Disease: Osteogenesis Imperfecta, Type I; Ehlers-danlos Syndrome, Type Vii, Autosomal Dominant; Osteogenesis Imperfecta, Type Ii; Ehlers-danlos Syndrome, Type I; Osteogenesis Imperfecta, Type Iii; Osteoporosis; Caffey Disease; Osteogenesis Imperfecta, Type Iv

size1 :

0.1 mg

price1 :

695 USD

more info or order :

MyBioSource product webpage