catalog number :
MBS534184
products full name :
VAChT antibody
products short name :
VAChT
products name syn :
Polyclonal VAChT; Anti-VAChT; Vesicular Acetycholine Transporter
other names :
vesicular acetylcholine transporter; Vesicular acetylcholine transporter; vesicular acetylcholine transporter; solute carrier family 18 (vesicular acetylcholine transporter), member 3; Solute carrier family 18 member 3
products gene name :
VAChT
other gene names :
SLC18A3; SLC18A3; VACHT; VACHT; VAChT
uniprot entry name :
VACHT_HUMAN
form :
Supplied as a lyophilized powder. Dissolve the antiserum in 50 - 100 ul distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.1% NaN3.
concentration :
50ul, lyohpilized
storage stability :
Ships ambient or refrigerated. Upon receipt store at 4 degree C. After reconstituted, store at 4 degree C while undiluted, or at -20 degree C in aliquots.
tested application :
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
app notes :
IF-FITC: 1:5,000-1:10,000
other info1 :
Immunogen: Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA. Biological Significance: The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylchiolinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse.
other info2 :
Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Synthetic peptide from the C-terminus of rat VACht conjugated to BSA.
products categories :
Neuroscience
products description :
Guinea Pig polyclonal VAChT antibody
ncbi acc num :
NP_003046.2
ncbi gb acc num :
NM_003055.2
ncbi mol weight :
56,903 Da
ncbi pathways :
Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Cholinergic Synapse Pathway (217716); Neuronal System Pathway (106513); Neurotransmitter Release Cycle Pathway (106518); Synaptic Vesicle Pathway (672457); Synaptic Vesicle Cycle Pathway (373900); Synaptic Vesicle Cycle Pathway (373890); Transmission Across Chemical Synapses Pathway (106516)
ncbi summary :
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008]
uniprot summary :
SLC18A3: Involved in acetylcholine transport into synaptic vesicles. Belongs to the major facilitator superfamily. Vesicular transporter family. Protein type: Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: membrane; integral to plasma membrane; plasma membrane. Molecular Function: acetylcholine transmembrane transporter activity. Biological Process: synaptic transmission; acetylcholine transport; neurotransmitter secretion