GAPDH antibody | MyBioSource MBS534081 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GAPDH antibody

catalog :

MBS534081

quantity :

0.1 ml

price :

180 USD

clonality :

polyclonal

host :

sheep

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS534081

products type :

Antibody

products full name :

GAPDH antibody

products short name :

GAPDH

products name syn :

Polyclonal GAPDH; Anti-GAPDH; Glyceraldehyde 3-Phosphate Dehydrogenase

other names :

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

products gene name :

GAPDH

other gene names :

GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH

uniprot entry name :

G3P_HUMAN

clonality :

Polyclonal

host :

Sheep

reactivity :

Human, Rabbit, E Coli

sequence length :

335

form :

Purified IgG fraction supplied in PBS, pH 7.2 with 0.1% NaN3.

storage stability :

Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles.

tested application :

ELISA (EIA), Western Blot (WB)

other info1 :

Biological Significance: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

other info2 :

Biohazard Information: This product contains sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. Immunogen: GAPDH antibody was raised in sheep using a 12 amino acid synthetic peptide (HQVVSSDFNSDT) representing the most conserved region of human and rat GAPDH conjugated with KLH as the immunogen.

products categories :

Proteases, Inhibitors, & Enzymes

products description :

Sheep polyclonal GAPDH antibody

products references :

Silica Crystals and Aluminum Salts Regulate the Production of Prostaglandin in Macrophages via NALP3 Inflammasome-Independent Mechanisms; Etsushi Kuroda, Ken J. Ishii, Satoshi Uematsu, Keiichi Ohata, Cevayir Coban, Shizuo Akira, Kosuke Aritake, Yoshihiro Urade, Yasuo Morimoto (Immunity: Volume: 34 Issue: 4 Page: 514-526 DOI: 10.1016/j.immuni.2011.03.019). The reduction of voluntary physical activity after poly I:C injection is independent of the effect of poly I:C-induced interferon-beta in mice; Takashi Matsumotoa, Hiroshi Takahashia, Daisuke Shivab, Noriaki Kawanishia, Michael J. Kremenika, Yasuko Katoc, Hiromi Yano (Physiology & Behavior: Volume: 93 Issue: 4-5 Page: 835-841 DOI: 10.1016/j.physbeh.2007.11.048). Calcineurin protects the heart in a murine model of dilated cardiomyopathy; Joerg Heinekea, b, Kai C. Wollertb, Hanna Osinskaa, Michelle A. Sargenta, Allen J. Yorka, Jeffrey Robbinsa, Jeffery D. Molkentin (Journal of Molecular and Cellular Cardiology: Volume: 48 Issue: 6 Page: 1080-1087 DOI: 10.1016/j.yjmcc.2009.10.012)

ncbi gi num :

7669492

ncbi acc num :

NP_002037.2

ncbi gb acc num :

NM_002046.4

uniprot acc num :

P04406

ncbi mol weight :

31,548 Da

ncbi pathways :

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Biosynthesis Of Antibiotics Pathway 1144997!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Gluconeogenesis Pathway 106204!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 413342

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

size :

0.1 ml

price :

180 USD

more info or order :

MyBioSource product webpage