AChE antibody | MyBioSource MBS531864 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

AChE antibody

catalog :

MBS531864

quantity :

0.1 ml

price :

425 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

immunohistochemistry, immunoprecipitation, western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS531864

products type :

Antibody

products full name :

AChE antibody

products short name :

AChE

products name syn :

Monoclonal AChE; Anti-AChE; Acetylcholinesterase

other names :

acetylcholinesterase; Acetylcholinesterase; acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase; acetylcholinesterase; N/A

products gene name :

AChE

other gene names :

ACHE; ACHE; YT; ACEE; ARACHE; N-ACHE; AChE

uniprot entry name :

ACES_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

403630

host :

Mouse

specificity :

Human

form :

Supplied as ascites with 0.1% NaN3.

storage stability :

Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles

tested application :

Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

app notes :

IHC: 1:200-1:1,000, WB: 1:500-1:1,000

other info1 :

Immunogen: AChE antibody was raised in mouse using AChE from human erythrocytes as the immunogen.

other info2 :

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

products categories :

Neuroscience; Monoclonal Antibodies

products description :

Mouse monoclonal AChE antibody. Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic nervous system, where its activity serves to terminate synaptic transmission.

ncbi gi num :

177975

ncbi acc num :

AAA68151.1

uniprot acc num :

P22303

ncbi mol weight :

67,796 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway 138006!!Acetylcholine Synthesis Pathway 198820!!Biogenic Amine Synthesis Pathway 198793!!Cholinergic Synapse Pathway 217716!!Glycerophospholipid Metabolism Pathway 82989!!Glycerophospholipid Metabolism Pathway 364!!Monoamine Transport Pathway 198851!!Neuronal System Pathway 106513!!Neurotransmitter Clearance In The Synaptic Cleft Pathway 106526!!Transmission Across Chemical Synapses Pathway 106516

ncbi summary :

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Ref.11 Ref.12 Ref.13 Ref.14. Catalytic activity: Acetylcholine + H2O = choline + acetate. Subunit structure: Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers . By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric. Subcellular location: Cell junction synapse. Secreted . By similarity. Cell membrane; Peripheral membrane protein . By similarity Ref.12 Ref.14. Isoform T: Nucleus. Note: Only observed in apoptotic nuclei. Ref.12 Ref.14Isoform H: Cell membrane; Lipid-anchor GPI-anchor; Extracellular side . By similarity Ref.12 Ref.14. Tissue specificity: Isoform H is highly expressed in erythrocytes. Ref.11. Polymorphism: ACHE is responsible for the Yt blood group system [. MIM:112100]. The molecular basis of the Yt(a)=Yt1/Yt(b)=Yt2 blood group antigens is a single variation in position 353; His-353 corresponds to Yt(a) and the rare variant with Asn-353 to Yt(b). Sequence similarities: Belongs to the type-B carboxylesterase/lipase family.

size :

0.1 ml

price :

425 USD

more info or order :

MyBioSource product webpage