antibody

Insulin antibody

MBS531836

1 mg

330 USD

monoclonal

mouse

nonconjugated

[M322213]

ELISA, enzyme immunoassay

Antibody

Insulin antibody

[Insulin]

[Monoclonal Insulin; Anti-Insulin]

[insulin; Insulin; insulin; insulin]

[INS; INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10]

INS_HUMAN

Monoclonal

IgG1

[M322213]

Mouse

Specific to human insulin. Cross react with human proinsulin, bovin insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.

110

Human

Insulin antibody was purified by chromatography on protein A Sepharose.

Liquid in PBS buffer, pH 7.4 containing 0.09% Sodium Azide.

6.4mg/ml

Store at 4 degree C. or -20 degree C for longer storage.

ELISA (EIA)

MBS531836 can be used as the conjugate antibody along with either 10-I30G or MBS531673 as the coating antibody. Alternatively, MBS531836 can be used as the coating antibody with MBS531719 as the conjugate antibody.

Biological Significance: Insulin is synthesized in significant quantities only in beta cells in the pancreas. It is secreted primarily in response to elevated blood concentrations of glucose. Insulin thus can regulate blood glucose and the body senses and responds to rise in blood glucose by secreting insulin. Affinity Constant: ~7.5 x 10 9 Liters/Mole

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Purified human insulin. Additional Remarks: Dectection of insulin in two-site enzyme immunoassay.

Hormones & Steroids

Mouse monoclonal Insulin antibody

386828

AAA59172.1

P01308

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Cardiac Progenitor Differentiation Pathway (712094)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Secreted, signal peptide; Hormone; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region. Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

1 mg

330 USD