antibody

Proinsulin antibody

MBS531673

1 mg

420 USD

monoclonal

mouse

nonconjugated

[M32237]

ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Proinsulin antibody

[Proinsulin]

[Monoclonal Proinsulin; Anti-Proinsulin]

[insulin preproprotein; Insulin; insulin; insulin]

[INS; INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10]

INS_HUMAN

Monoclonal

IgG1 kappa

[M32237]

Mouse

Reacts with the connection site of C-Peptide and the C-end of the B-chain of proinsulin. Does not react with human, bovine or porcine insulin.

110

Human

Proinsulin antibody was purified by chromatography on protein A Sepharose.

Protein A purified, supplied in PBS buffer, pH7.4 with 0.1% NaN3.

6.5mg/ml

Store at 4 degree C or -20 degree C for longer storage.

ELISA (EIA), Immunohistochemistry (IHC)

MBS531673 can be used as the coating antibody with MBS531836 as the conjugate antibody. This anitbody is suitable for Flow cytometry and immunohistochemistry applications on frozen sections. Optimal conditions to be determined by end user.

Biological Significance: Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of the pancreas. In humans, proinsulin is encoded by the INS gene. Proinsulin is synthesized in the endoplasmic reticulum, where it is folded and its disulfide bonds are oxidized. It is then transported to the Golgi apparatus where it is packaged into secretory vesicles, and where it is processed by a series of proteases to form mature insulin.

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Immunogen: Proinsulin antibody was raised in mouse using human proinsulin as the immunogen.

Hormones & Steroids

Mouse monoclonal Proinsulin antibody

4557671

NP_000198.1

NM_000207.2

P01308

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Cardiac Progenitor Differentiation Pathway (712094)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Hormone; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region. Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; positive regulation of cell differentiation; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

1 mg

420 USD