MyBioSource

antibody

Troponin I antibody (Cardiac)

MBS530749

1 mg

560 USD

monoclonal

mouse

human

immunohistochemistry, western blot, enzyme immunoassay, immunoprecipitation, ELISA

MBS530749

Antibody

Troponin I antibody (Cardiac)

Troponin I (Cardiac)

Monoclonal Troponin I (Cardiac); Anti-Troponin I (Cardiac)

troponin I, cardiac muscle; Troponin I, cardiac muscle; troponin I, cardiac muscle; troponin I type 3 (cardiac); Cardiac troponin I

TNNI3; TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; TNNC1

TNNI3_HUMAN

Monoclonal

IgG2b

M805142

Mouse

Human

Troponin I antibody (Cardiac) was purified by chromatography on protein A Sepharose.

Protein A purified IgG supplied in PBS, pH 7.4, 0.1 % NaN3.

Store at 4 deg C.

ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

10-T79C can be used in a sandwich immunoassay as a coating antibody in conjunction with 10-T79J as the conjugate antibody. Alternatively, 10-7965, 10-T79F, or 10-T79H can be used in a sandwich immunoassay as a coating antibody in conjunction with 10-T79C as the conjugate antibody.

Immunogen: Troponin I antibody (Cardiac) was raised in mouse using animo acid residues 41-49 of cTnI as the immunogen.

Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Cardiac Markers; Monoclonal Antibodies

Mouse monoclonal Troponin I antibody (Cardiac) . Human troponin I is presented in cardiac muscle tissue by a single isoform with molecular weight 23876 Da and it consists of 209 amino acid residues. cTnI molecule contains two serines in the 22 and 23 positions. Both amino acid residues can be phosphorylated in vivo by protein kinase A, so four forms of protein - one dephospho, two monophospho and one bisphospho - can coexist in the cell.

151101270

NP_000354.4

NM_000363.4

P19429

24,008 Da

Cardiac Muscle Contraction Pathway 93344!!Cardiac Muscle Contraction Pathway 93992!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285!!Hypertrophic Cardiomyopathy (HCM) Pathway 114229!!Hypertrophic Cardiomyopathy (HCM) Pathway 106591!!Muscle Contraction Pathway 106261!!Striated Muscle Contraction Pathway 106262!!Striated Muscle Contraction Pathway 198903

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Subunit structure: Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Ref.9 Ref.10. Post-translational modification: Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction . By similarity. Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Ref.5 Ref.6 Ref.7 Ref.8 Ref.10. Involvement in disease: Defects in TNNI3 are the cause of familial hypertrophic cardiomyopathy type 7 (CMH7) [. MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Ref.13 Ref.14 Ref.16 Ref.18 Ref.20Defects in TNNI3 are the cause of familial restrictive cardiomyopathy type 1 (RCM1) [. MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Ref.15Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [. MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Ref.19Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [. MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Sequence similarities: Belongs to the troponin I family.

1 mg

560 USD