protein

Ebola Virus NP protein

MBS5304199

0.1 mg

210 USD

Recombinant Protein

Ebola Virus NP protein

[Ebola Virus NP]

[Ebola Virus Nucleoprotein; Ebola protein; Ebola virus antigen; Zaire Ebola nucleoprotein antigen; Zaire NP protein; Ebola Zaire nucleoprotein antigen; Ebola virus protein]

[nucleoprotein; nucleoprotein]

[NP]

Viral

739

> 90% pure. Purified using proprietary chromatographic techniques

Liquid in 50mM Tris,pH 8, 200 mM NaCI, 8M urea, 1 mM EDTA, 2mM DTT.

1.0 mg/mL

4°C shipping only. Upon receipt store at -70°C.

SDS-PAGE, Western Blot (WB)

Ebola Virus antibody can be used to detect this antibody

SDS-PAGE

Western Blot (WB)

Western Blot(WB)

Protein Type: Recombinant. Residues: 1-739 aa (GenBank: AHX24646.1). Implication In Disease: Typically, Ebola virus infection runs its course within 14 to 21 days. Infection initially presents with nonspecific flu-like symptoms such as fever, myalgia, and malaise. As the infection progresses, patients exhibit severe bleeding and coagulation abnor. Post Translational Modifications: Ebola nucleoprotein is Phosphorylated and O-glycosylated by the host organisms cells. Biological Significance: The Ebola Virus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation. Expression System: E Coli. Tag/Conjugate: His tag

Expression: The viral RNA dependent RNA polymerase binds the encapsidated genome at the leader region, then sequentially transcribes each genes by recognizing start and stop signals flanking viral genes. mRNAs are capped and polyadenylated by the L protein during synthesis. The primary product of the unedited transcript of GP gene yields a smaller non-structural glycoprotein sGP which is efficiently secreted from infected cells. RNA editing allows expression of full-length GP. Implications in Disease: Typically, Ebola virus infection runs its course within 14 to 21 days. Infection initially presents with nonspecific flu-like symptoms such as fever, myalgia, and malaise. As the infection progresses, patients exhibit severe bleeding and coagulation abnormalities, including gastrointestinal bleeding, rash, and a range of hematological irregularities, such as lymphopenia and neutrophilia. Cytokines are released when reticuloendothelial cells encounter virus, which can contribute to exaggerated inflammatory responses that are not protective. Damage to the liver, combined with massive viremia, leads to disseminated intravascular coagulopathy. The virus eventually infects microvascular endothelial cells and compromises vascular integrity. The terminal stages of Ebola virus infection usually include diffuse bleeding, and hypotensive shock accounts for many Ebola virus fatalities. Sequence information: The Ebola nucleoprotein is a homooligomer found on the Ebola virus. It homomultimerizes to form the nucleocapsid. At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. The predicted coding region is 2217 bases in length and encodes a protein that contains 739 amino acids, with a calculated molecular weight of 83.3 kDa. The protein has an approximate net charge of -30 and can be divided into a hydrophobic N-terminal half and a hydrophilic and highly acidic C-terminal half.

Infectious Disease; Conjugated Proteins

Purified Recombinant Ebola Nucleoprotein antigen (Zaire strain)

22789223

NP_690580

NC_004161.1

87 kDa

0.1 mg

210 USD