TRIM21 protein (His tag) | MyBioSource MBS5303811 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TRIM21 protein (His tag)

catalog :

MBS5303811

quantity :

0.1 mg

price :

170 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS5303811

products type :

Recombinant Protein

products full name :

TRIM21 protein (His tag)

products short name :

[TRIM21]

products name syn :

[52 KDa Ribonucleoprotein Autoantigen Ro; Ro-52 protein; Trim21 protein; RNF81 protein; RO52 protein; SSA protein; SSA1 protein; TRIM 21 protein]

other names :

[Trim21; Trim21 protein; E3 ubiquitin-protein ligase TRIM21; tripartite motif-containing 21; Trim21 protein]

products gene name :

[TRIM21]

other gene names :

[Trim21; Trim21; Ro52; Ssa1]

uniprot entry name :

Q921V6_MOUSE

host :

E.coli

purity :

> 90% pure . Purified using propietary chromatographic techniques.

form :

Liquid in 50mM Tris, pH 8.5, with 150mM NaCl, 0.02% SDS, 10% glycerol, 1mM EDTA, and 2mM DTT.

concentration :

1mg/ml

storage stability :

Shipping: Ships on dry ice. Upon receipt store at -70°C. Avoid repeated freeze-thaw cycles.

image1 heading :

Test Data

other info1 :

Purification Method: Purified using proprietary chromatographic techniques. Protein Type: Recombinant. Biological Significance: TRIM21 is an intracellular antibody effector in the intracellular antibody-mediated proteolysis pathway. It binds to immunoglobulin G as well as immunoglobulin M on antibody marked non-enveloped virions which have infected the cell.

other info2 :

Residues: 1-285aa. Tag/Conjugate: His tag. Expression: As a component of the proteolysis pathway, TRIM21 is expressed in most human tissues. Tissue and cell localizatin: TRIM21 is mainly found in the cytoplasm and the nucleus. Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung. Implications in Disease: TRIM21 interacts with autoantigens in patients with Sjogren's Syndrome and systematic lupus erythematosus. Post-Translational Modifications: TRIM21 is autoubiquitinated which does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization. Ubiquitination occurs at Lys260.

products categories :

Immunology; Conjugated Proteins

products description :

Purified recombinant TRIM21 (Ro52) protein (His tag)

ncbi gi num :

71060001

ncbi acc num :

CAJ18544.1

uniprot acc num :

Q921V6

ncbi mol weight :

31 kDa

ncbi pathways :

Adaptive Immune System Pathway (1168853); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1168871); Class I MHC Mediated Antigen Processing Presentation Pathway (1168870); Cytokine Signaling In Immune System Pathway (1168969); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1168940); Immune System Pathway (1168852); Innate Immune System Pathway (1168881); Interferon Signaling Pathway (1168970); Interferon Gamma Signaling Pathway (1168973); Regulation Of Innate Immune Responses To Cytosolic DNA Pathway (1168949)

uniprot summary :

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ( Thr- 187 phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase. Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; intracellular; ribonucleoprotein complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity. Biological Process: protein monoubiquitination; negative regulation of viral transcription; protein autoubiquitination; protein polyubiquitination; positive regulation of virion penetration into host cell; protein destabilization; inhibition of NF-kappaB transcription factor; protein ubiquitination; positive regulation of cell cycle; innate immune response; positive regulation of transcription factor activity; cell cycle

size1 :

0.1 mg

price1 :

170 USD

size2 :

1 mg

price2 :

595

more info or order :

MyBioSource product webpage