product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-His Tag (HIS.H8) Monoclonal Antibody
catalog :
MBS525001
quantity :
0.05 mg
price :
185 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[HIS.H8]
application :
western blot, ELISA, immunoprecipitation, enzyme immunoassay, dot blot
more info or order :
image
image 1 :
Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
image 2 :
Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)
image 3 :
Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
product information
catalog number :
MBS525001
products type :
Antibody
products full name :
Anti-His Tag (HIS.H8) Monoclonal Antibody
products short name :
[His Tag]
products name syn :
[Anti-His Tag]
clonality :
Monoclonal
isotype :
IgG2b
clone :
[HIS.H8]
host :
Mouse
specificity :
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
purity :
Protein A affinity chromatography from mouse ascites fluid
form :
1 mg/ml in 10mM PBS, pH 7.2, with 0.05% NaN3
storage stability :
Store at -20 degree C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 degree C for several days to weeks.
tested application :
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
app notes :
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. IP: 1:500-200 dilution. For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test
image1 heading :
Testing Data
image2 heading :
Testing Data #2
image3 heading :
Testing Data #3
other info1 :
Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH
other info2 :
Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins
products categories :
Epitope Tag mAbs
size1 :
0.05 mg
price1 :
185 USD
size2 :
0.1 mg
price2 :
225
size3 :
0.2 mg
price3 :
305
size4 :
1 mg
price4 :
760
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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