catalog number :
MBS524315
products full name :
Anti-Bovine RBC, IgM Fraction, (Polyclonal) (rabbit IgM)
products short name :
[RBC]
other names :
[Ribulose bisphosphate carboxylase; Ribulose bisphosphate carboxylase; ribulose bisophosphate carboxylase]
products gene name :
[RBC]
other gene names :
[TK_RS11535; rbcL; RuBisCO]
uniprot entry name :
RBL_THEKO
form :
5 mg of IgM fraction in sterile phosphate buffered saline
storage stability :
Divide into useful aliquots and store at 2-8°C .
tested application :
This reagent is suitable for use in cytotoxic assays.
other info1 :
Preservatives: 0.05% Sodium Azide (NaN 3 )
other info2 :
Test Type:
Hemmagglutaination:. Test Cell Suspension: 100 uL of washed 1% fixed bovine red blood cells in saline.
Product Tested: 100 ul of the above listed product and lot diluted in 1:100 heat inactivated normal rabbit serum in saline.
Incubation: 37°C for 20 mins, in U-bottom microtiter plates . 20°C for 2-4 hrs. for cell settling pattern.
Titer: The highest dilution of the product that yields agglutination.
Results: Titer of 1:100.
Ouchterlony.
Sample size: 10uL.
Distance between cells 5mm.
Test time : 72 hours.
Antiserum tested: Goat anti-rabbit IgM, goat anti-rabbit IgG
products categories :
Bovine
products description :
MBS524315 is rabbit IgM directed against Bovine red blood cells. This IgM fraction was isolated from a high titer antiserum by salt fractionation, ion exchange and gel filtration chromatography. It was tittered against its immunogens, sterile filtered and analyzed for immunoglobulin purity and protein concentration. This IgM fraction contains agglutinating and lysing antibodies and can be used for these procedures.
ncbi pathways :
Carbon Metabolism Pathway (816423); Carbon Metabolism Pathway (817567); Glyoxylate And Dicarboxylate Metabolism Pathway (22014); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Metabolic Pathways (132199); Microbial Metabolism In Diverse Environments Pathway (179988); Adenosine Nucleotides Degradation IV Pathway (139586)
uniprot summary :
Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA) (PubMed:9988755, PubMed:10512715). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase (PubMed:17303759).