product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-Sheep RBC (red blood cells), (rabbit IgM)
catalog :
MBS524107
quantity :
1 mL
price :
220 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
more info or order :
product information
catalog number :
MBS524107
products type :
Antibody
products full name :
Anti-Sheep RBC (red blood cells), (rabbit IgM)
products short name :
RBC (red blood cells)
products name syn :
RBC (red blood cells), (rabbit IgM); HAEMOLYSIN: RABBIT ANTI-SHEEP RED BLOOD CELL STROMA
products gene name :
RBC
isotype :
Rabbit lgM
host :
Rabbit
reactivity :
Sheep
specificity :
RBC
storage stability :
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropritate for single usage. Avoid repeated freeze/thaw cycles.
app notes :
For complement fixation and complement dependent haemolytic studies using sheep red blood cells as targets.
other info1 :
Sterility: This reagent is not sold as sterile but may be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.22um Millipore filter (or equivalent). Haemolytic Properties: for haemolytic assays using 1-2% srbc's the suggested dilution is 1:400
other info2 :
Immunization: Rabbit anti-sheep red blood cell stroma. Presentation: 1.0 ml in PBS+ 0.02% NaN3. Method : METHOD FOR DETERMINING HEMOLYTIC TITRE OF
HAEMOLYSIN ANTI-SHEEP RED BLOOD CELL ANTISERUM:. 1. Wash and pack sheep red blood cells in VBD media. Dilute to 1 % in VBD.
2. Using a 96-well round-bottom microtitre plate, add the antibody to a final. concentration of 1:2 and dilute serially to a dilution of 1:2 million using doubling dilutions. 3. Add cells and incubate for 30 minutes at room temperature. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in MBSs Hemo-Lo Guinea Pig Complement diluted to 1:20 in VBD. 6. Incubate for 60 minutes at 37°C. 7. Read plate by visual inspection to determine hemolytic titre.
products description :
Haemolysin is a serum containing a high level of antibody against sheep erythrocytes. When this antiserum is combined with erythrocytes in suspension, the erythrocytes are said to be sensitized (ie. in the presence of free complement they will lyse). An euglobin precipitation was performed on the antiserum in order to purity the IgM fraction.
size1 :
1 mL
price1 :
220 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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