antibody

Anti-Human CD200, Purified, (Clone: 1B9) (Rat IgG2a)

MBS520896

0.25 mg

385 USD

monoclonal

rat

nonconjugated

[1B9]

ELISA, flow cytometry, enzyme immunoassay

Antibody

Anti-Human CD200, Purified, (Clone: 1B9) (Rat IgG2a)

[CD200]

[CD200 antigen, isoform CRA_a; OX-2 membrane glycoprotein; CD200 molecule]

[CD200]

[CD200; MRC; MOX1; MOX2; OX-2]

m

IgG2a

[1B9]

Rat

Human

This antibody is specific for human CD200.

Purified

1.0 mg/ml

Store at 4°C. DO NOT FREEZE APC, PE, AF488, and AF647 conjugates. For long term storage ( Purified, LE, Biotin and FITC ), aliquot and freeze unused portion -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

ELISA (EIA), Flow Cytometry (FC)

Tissue Distribution by Flow Cytometry Analysis: . Cell Concentration: 1x10 6 cells per tests. Antibody Concentration Used: 0.1 ug/10 6 cells. Cell Source: Human PBLs. Percentage of cells stained above control: 16.4 %. *For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.

Flow Cytometry

Immunogen: Human CD200-Fc fusion protein. Conjugate: Unconjugated

Presentation: Purified: Purified IgG buffered in PBS AND 0.02% NaN 3 . (Purified via Protein G Chromatography). For maximum recovery of contents, spin down tube before use. LE: Purified Ig buffered in PBS, no preservative, 0.2um sterile filtered. (Purified from cell culture supernatant via Protein G Chromataopgraphy). Biotin, FITC, PE, APC, AF488 and AF647: Biotin/FITC/PE/APC/AF488/AF647 conjugated IgG buffered in PBS, 0.02% NaN 3 and EIA grade BSA as a stabiizing protein to bring total protein concentration to 4-5 mg/ml.

CD200, also known as OX-2, is a type-1 membrane glycoprotein that belongs to the immunoglobin superfamily (IgSF). It's expressed on several tissues including thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of CD34+ progenitor cells, and at low levels on some smooth muscle and B lymphocytes. CD200 is not expressed on NK cells, monocytes, granulocytes, or platelets. CD200 co-stimulates T-cell proliferation and may regulate myeoid cell activity in a variety of tissues. Additionally, interaction of CD200 with its receptor on macrophages induces a downregulation of macrophage activity in a variety of tissues, suggesting a regulatory function.

119600082

EAW79676.1

Adaptive Immune System Pathway (366160); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

0.25 mg

385 USD