Anti-Rat CD8a PE-Cy5 (Clone OX-8) (mouse IgG1) | MyBioSource MBS520546 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Rat CD8a PE-Cy5 (Clone OX-8) (mouse IgG1)

catalog :

MBS520546

quantity :

0.1 mg

price :

680 USD

clonality :

monoclonal

host :

mouse

conjugate :

PE-Cy5

clone name :

OX-8

reactivity :

human, rat

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS520546

products type :

Antibody

products full name :

Anti-Rat CD8a PE-Cy5 (Clone OX-8) (mouse IgG1)

products short name :

CD8a PE-Cy5

products name syn :

CD8a PE-Cy5 (Clone OX-8) (mouse IgG1); PE-Cy5 Anti-Rat CD8 Monoclonal Antibody

other names :

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2

products gene name :

CD8a PE-Cy5

other gene names :

CD8A; CD8A; CD8; MAL; p32; Leu2; MAL

uniprot entry name :

CD8A_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1

clone :

OX-8

host :

Mouse

reactivity :

Rat

sequence length :

235

specificity :

CD8a (Rat CD8a)

form :

PE-Cy5

concentration :

Antibody Concentration: 0.2 mg/ml

storage stability :

Store at 4 degree C. DO NOT FREEZE. Avoid prolonged exposure to light. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Check label for expiry date.

tested application :

Immunohistochemistry (IHC)

other info2 :

Presentation: 100 ug PE-Cy5 conjugated Ig buffered in PBS, 0.09% sodium azide (NaN3) and 0.5% BSA as as stabilizer.

products description :

anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) MBS520546 labels all peripheral T cells that are unlabelled by the (W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) MBS520546 and can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being (W3/13) which labels all T cells, as well as (W3/25) and MBS520546(MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. Applications Include: Flow cytometry

ncbi gi num :

19344022

ncbi acc num :

AAH25715.1

uniprot acc num :

P01732

ncbi mol weight :

25,729 Da

ncbi pathways :

Adaptive Immune System Pathway 366160!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL12-mediated Signaling Events Pathway 137922!!Immune System Pathway 106386

ncbi summary :

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not . By similarity. Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

size :

0.1 mg

price :

680 USD

more info or order :

MyBioSource product webpage