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antibody

Anti-Mouse CD44, Purified (Clone IM7.8.1) (rat IgG2b)

MBS520523

0.2 mg

270 USD

monoclonal

rat

human, mouse

flow cytometry, FACS, immunoprecipitation, enzyme immunoassay, ELISA, western blot, immunohistochemistry

MBS520523

Antibody

Anti-Mouse CD44, Purified (Clone IM7.8.1) (rat IgG2b)

CD44

CD44, Purified (Clone IM7.8.1) (rat IgG2b); Purified Anti-Mouse CD44 Monoclonal Antibody

CD44; CD44 antigen; CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; CD44 molecule (Indian blood group); CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I

CD44

CD44; CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I

CD44_HUMAN

Monoclonal

Rat IgG2b

IM7.8.1

Rat

Mouse

43

CD44 (Ly 24, Pgp-1) (Mouse CD44)

Purified

Antibody Concentration: 0.2 mg/ml

Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)

Presentation: 200 ug purified Ig buffered in PBS and 0.05% sodium azide 0.1% (NaN3). FLOW CYTOMETRY ANALYSIS: Method: . 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x 10^7 cells/ml in media A. Add 50ul of this suspension to each tube (each tube will then contain 1 x 10^6 cells, representing 1 test). 4. To each tube, add ~1.0ug*. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4 degree C. 7. Wash 2 times at 4 degree C. 8. Add 100 J11 of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1:500 dilution. 9. Incubate the tubes at 4 degree C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4 degree C in media B. 11. Resuspend the cell pellet in 50 ul ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media . A. Phosphate buffered saline (pH 72) + 5% normal serum of host species + sodium azide ( 100 ul of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls). N.B. Appropriate contml samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.

anti-mouse CD44 monoclonal antibody reacts with all isoforms of CD44 (also known as Pgp-1, Ly-24) glycoprotein. By flow cytometry, the main cellular reactivities are B cellls, monocytes, macrophages and variable subsets of thymocyctes and peripheral T-cells.

685122

AAB30429.1

P16070

81,538 Da

Cytokine Signaling In Immune System Pathway 366171!!Disease Pathway 530764!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!Extracellular Matrix Organization Pathway 576262!!Glycosaminoglycan Metabolism Pathway 645297!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Function: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Ref.31. Subunit structure: Interacts with PKN2 . By similarity. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Ref.41. Subcellular location: Membrane; Single-pass type I membrane protein. Note: Colocalizes with actin in membrane protrusions at wounding edges . By similarity. Tissue specificity: Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. Domain: The lectin-like LINK domain is responsible for hyaluronan binding . By similarity. Post-translational modification: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. Ref.28N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Ref.28 Ref.39Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. Ref.26 Ref.27. Polymorphism: CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46. Sequence similarities: Contains 1 Link domain.

0.2 mg

270 USD