catalog number :
MBS520461
products full name :
Anti-Mouse CD34, FITC (Clone MEC14.7) (rat IgG2a)
products short name :
CD34
products name syn :
CD34, FITC (Clone MEC14.7) (rat IgG2a); Anti-Mouse CD34 Monoclonal Antibody
other names :
CD34; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule
products gene name :
CD34
other gene names :
CD34; CD34
uniprot entry name :
CD34_HUMAN
specificity :
CD34 (mucosialin; sialomucin) (Mouse CD34)
concentration :
0.1 mg/ml
storage stability :
For all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Ascites, Purified, Biotin, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
tested application :
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
other info2 :
Presentation: Biotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
products description :
progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. The antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells. It is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. This clone is suitable for use in ELISA, immunofluorescence, immunohistochemistry on frozen4 and paraffin sections2,3, Western Blot, immunoprecipitation, flow cytometry and cell separation
ncbi acc num :
AAB25223.1
ncbi mol weight :
40,716 Da
ncbi pathways :
Adaptive Immune System Pathway (366160); C-MYB Transcription Factor Network Pathway (138073); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)
ncbi summary :
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
uniprot summary :
Function: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Developmental stage: On early hematopoietic progenitor cells. Post-translational modification: Highly glycosylated.Phosphorylated on serine residues by PKC. Ref.10. Sequence similarities: Belongs to the CD34 family. Sequence caution: The sequence AAA03181.1 differs from that shown. Reason: Erroneous initiation.