catalog number :
MBS520283
products full name :
Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b)
products short name :
Erythroid Cell (Ly 76)
products name syn :
Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b); Biotin Anti-Mouse Erythroid Cell (Ly-76) Monoclonal Antibody
specificity :
Erythroid Cells (Ly-76) (Mouse Erythroid Cells (Ly-76))
form :
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from supernatant via Protein G Chromatography).)
concentration :
Antibody Concentration: 0.1 mg/ml
storage stability :
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
tested application :
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
other info1 :
Immunogen: Day 14 BALB/c fetal liver cells. Donor: Wistar rat spleen. Fusion Partner: X63.Ag8.653 myloma cells
other info2 :
Presentation: 100 ug or 300ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
products description :
anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes. TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells. This antibody is suitable for use in flow cytometry. It has also be reported to work in Western Blotting, immunoprecipitation and immunohistochemistry (frozen and paraffin).