antibody

Anti-Rat CD8a Biotin (Clone OX-8) (mouse IgG1)

MBS520223

0.1 mg

245 USD

monoclonal

mouse

biotin

OX-8

human, rat

western blot, immunohistochemistry, flow cytometry, FACS

Antibody

Anti-Rat CD8a Biotin (Clone OX-8) (mouse IgG1)

CD8a

CD8a Biotin (Clone OX-8) (mouse IgG1); Biotin Anti-Rat CD8a Monoclonal Antibody

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2

CD8a

CD8A; CD8A; CD8; MAL; p32; Leu2; MAL

CD8A_HUMAN

Monoclonal

Mouse IgG1

OX-8

Mouse

Rat

235

CD8a (Rat CD8a)

Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))

Antibody Concentration: 0.1 mg/ml

Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Immunogen: High molecular weight rat thymocyte glycoproteins. Donor: BALB/c spleen. Fusion Partner: P3-NSI-1-Ag4 (NSI/1)

Presentation: 100 ug or 500 ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) MBS520223 labels all peripheral T cells that are unlabelled by the (W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) MBS520223 can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being (W3/13) which labels all T cells, as well as (W3/25) and (MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).

19344022

AAH25715.1

P01732

25,729 Da

Adaptive Immune System Pathway 366160!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL12-mediated Signaling Events Pathway 137922!!Immune System Pathway 106386

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not . By similarity. Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.1 mg

245 USD