Anti-Mouse CD8a (Ly 2.2), FITC (Clone AD4(15)) (mouse IgM) | MyBioSource MBS520221 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Mouse CD8a (Ly 2.2), FITC (Clone AD4(15)) (mouse IgM)

catalog :

MBS520221

quantity :

0.1 mg

price :

220 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

AD4(15)

reactivity :

human, mouse

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS520221

products type :

Antibody

products full name :

Anti-Mouse CD8a (Ly 2.2), FITC (Clone AD4(15)) (mouse IgM)

products short name :

CD8a (Ly 2.2)

products name syn :

CD8a (Ly 2.2), FITC (Clone AD4(15)) (mouse IgM); FITC-Anti-Mouse CD8a (Ly 2.2) Monoclonal Antibody

other names :

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2

products gene name :

CD8a (Ly 2.2)

other gene names :

CD8A; CD8A; CD8; MAL; p32; Leu2; MAL

uniprot entry name :

CD8A_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgM

clone :

AD4(15)

host :

Mouse

reactivity :

Mouse

sequence length :

235

specificity :

CD8a (Ly 2.2) (Mouse CD8a (Ly2.2))

form :

FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from acites via euglobin).)

storage stability :

Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.

tested application :

Immunohistochemistry (IHC)

other info1 :

Immunogen: C57BL/6. Donor: B6-Ly-2a. Fusion Partner: Myeloma line P3/X63Ag8

other info2 :

Presentation: 100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

products description :

anti-mouse CD8a (Ly 2.2) monoclonal antibody reacts with a subpopulation of T-lymphocytes from mouse strains expressing the Ly-2.2 phenotype but does not react with lymphocytes from strains expressing the Ly-2.1 phenotype. This clone has been shown to work in both cytotoxicity assays and flow cytometry.

ncbi gi num :

19344022

ncbi acc num :

AAH25715.1

uniprot acc num :

P01732

ncbi mol weight :

25,729 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (106386)

ncbi summary :

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not . By similarity. Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

size1 :

0.1 mg

price1 :

220 USD

size2 :

0.3 mg

price2 :

350

more info or order :

MyBioSource product webpage