antibody

Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b)

MBS520216

0.25 mg

270 USD

monoclonal

rat

nonconjugated

YTS 169 AG 101 HL

human, mouse

immunohistochemistry, flow cytometry, FACS

Antibody

Anti-Mouse CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b)

CD8a (Ly 2)

CD8a (Ly 2) Purified (Clone YTS 169.4) (rat IgG2b); Anti-Mouse CD8a (Ly 2) Monoclonal Antibody

CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2

CD8a (Ly 2)

CD8A; CD8A; CD8; MAL; p32; Leu2; MAL

CD8A_HUMAN

Monoclonal

Rat IgG 2 b

YTS 169 AG 101 HL

Rat

Mouse

235

CD8a (Ly 2; Lyt 2) (Mouse CD8a (Ly 2)). Specifications: . Clone: YTS 169AG 101HL. Hybridoma Production: . Immunization: Immunogen: Mouse Ly-2 thymocytes. Donor: (Lou x DA) F1 rat. Fusion Partner: Myeloma Y3/Ag1.2.3. Specificity: Mouse CD8a (Ly 2). Strains Tested: C57BL/6, BALB/c, AKR/J, C3H/He. Positive: C57BL/6, BALB/c, AKR/J, C3H/He. Negative: None

Purified

1.0 mg/ml

Store Ascites at -20 degree C. For all other formats, store at 4 degree C. DO NOT FREEZE APC and PE conjugates. For long term storage (Purified, Biotin, FITC and No Azide), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC), Cytotoxic Assay (CA), Flow Cytometry (FC/FACS), In-Vivo Depletion (IV)

Immunogen: Mouse Ly-2 thymocytes. Donor: (Lou x DA) F1 rat. Fusion Partner: myeloma Y3/Ag1.2.3

Presentation: Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.

purified anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology1. It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro. This antibody has been reported to be suitable for use in immunohistochemistry on PFA-fixed paraffin-embedded tissue sections (antigen retrieval is required).7 This clone has been reported to work in immunohistochemistry6 (frozen sections).

19344022

AAH25715.1

P01732

25,729 Da

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (106386)

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not . By similarity. Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

0.25 mg

270 USD