catalog number :
MBS520165
products full name :
Anti-Rat CD45RC PE (Clone OX-22) (mouse IgG1)
products short name :
CD45RC
products name syn :
CD45RC PE (Clone OX-22) (mouse IgG1); PE Anti-Rat CD45RC Monoclonal Antibody
products gene name :
CD45RC
specificity :
CD45RC (Rat CD45RC (L-CA, high molecular weight form))
form :
PE (R-PE conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
concentration :
Antibody Concentration: 0.1 mg/ml
storage stability :
For all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
tested application :
Immunohistochemistry (IHC)
other info1 :
Immunogen: Phytohaemagglutinin-activated rat lymph node cells. Fusion Partner: P3-NSI-1-Ag4 (NS1/1). Immunocyte Donor: BALB/c spleen
other info2 :
Presentation: 50 ug or 200 ug R-PE conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
products description :
Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes gives one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa and B cells; one broad band at 240 kDa. T helper and T cytotoxic cell subsets show the same 4 bands with some differences in the proportions of each. MBS520165 reacts with the high molecular weight form of the leukocyte common antigen (2) which includes the 240 kDa form on B cells as well as a subfraction of the 190. 200 and 220 kDa forms, but not with the 180 kDa form of T cells or thymocytes. (5) Thus, MBS520165 reacts with B cells, cytotoxic T cells and helper cells for T cell responses but not with thymocytes or helper T cells for in vivo antibody responses. (5) Consequently, this antibody can be used to identifY B cells, all CD8+ve T cells, 75% CD4+ve T cells and 50% Bone Marrow cells. This, antibody also divides rat CD4+ve T cells into two phenotypically distinct populations differing in their helper activities (6,7).