antibody

Anti-Rat CD44 Purified (Clone OX-49) (mouse IgG2a)

MBS520130

0.25 mg

250 USD

monoclonal

mouse

nonconjugated

OX-49

human, rat

immunohistochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

Anti-Rat CD44 Purified (Clone OX-49) (mouse IgG2a)

CD44

CD44 Purified (Clone OX-49) (mouse IgG2a); Purified Anti-Rat CD44 Monoclonal Antibody

CD44; CD44 antigen; CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; CD44 molecule (Indian blood group); CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I

CD44

CD44; CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I

CD44_HUMAN

Monoclonal

Mouse IgG2a

OX-49

Mouse

Rat

43

CD44 (Pgp-1) (Rat CD44)

Purified (Purified Ig buffered in PBS and 0.02% NaN3 (Purified from supernatant via Protein G Chromatography).)

Antibody Concentration: 1.0 mg/ml

Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Immunogen: T cell blasts. Donor: BALB/c spleen. Fusion Partner: myeloma cell line NSO/1

Presentation: 250 ug or 500 ug purified Ig buffered in PBS and 0.02% NaN3.

anti-rat CD44 (OX-49) monoclonal antibody recognizes rat CD44 (Pgp-1), also called CD44H. This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. The OX-49 antibody binds extracellularly to the standard (S) form on rat leukocytes, but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form). This antibody is suitable for immunoprecipitation, flow cytometry, Western Blotting and immunohistochemisty on frozen and paraffin sections.

685122

AAB30429.1

P16070

81,538 Da

Cytokine Signaling In Immune System Pathway (366171); Disease Pathway (530764); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (576262); Glycosaminoglycan Metabolism Pathway (645297); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Function: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Ref.31. Subunit structure: Interacts with PKN2 . By similarity. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Ref.41. Subcellular location: Membrane; Single-pass type I membrane protein. Note: Colocalizes with actin in membrane protrusions at wounding edges . By similarity. Tissue specificity: Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. Domain: The lectin-like LINK domain is responsible for hyaluronan binding . By similarity. Post-translational modification: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. Ref.28N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Ref.28 Ref.39Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. Ref.26 Ref.27. Polymorphism: CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46. Sequence similarities: Contains 1 Link domain.

0.25 mg

250 USD

0.5 mg

345