catalog number :
MBS520112
products full name :
Anti-Mouse CD4 No Azide (Clone YTS 191.1) (rat IgG2b)
products short name :
[CD4 No Azide]
products name syn :
[Purified Anti-Mouse CD4 (L3/T4) Monoclonal Antibody]
other names :
[CD4; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); OTTHUMP00000238897; T-cell surface antigen T4/Leu-3; CD4 molecule]
products gene name :
[CD4]
other gene names :
[CD4; CD4; CD4mut; CD4 receptor]
uniprot entry name :
Q13969_HUMAN
specificity :
Mouse CD4 (L3/T4)
form :
Purified Ig buffered in PBS, and 0.02% NaN3.(Purified from ascitic fluid via Protein G chromatography). For maximum recovery of contents, spin down tube before use.
storage stability :
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
tested application :
Flow Cytometry
app notes :
Cytotoxic depletion and Flow Cytometry. This clone has been reported to work in immunofluorescence and in-vivo cell depletion.
other info1 :
Hybridoma Production: Immunization: Immunogen: murine thymocytes. Donor: (LOU x DA) F1 rats. Fusion Partner: Y3/Ag 1.2.3
other info2 :
Presentation: 1 mg purified Ig buffered in PBS, no preservative, 0.2 um filtered
products description :
The CD4 (L3/T4) antigen is thought to be the murine equivalent of the human Leu3/OKT4 antigen. This T cell surface molecule appears to be expressed by the helper/inducer subset of murine T cells and by delayed hypersensitivity T cells but not by cytotoxic T cells or their precursors. CD4 (L3/T4) and CD8a (Ly 2) have been shown to be present on mutually exclusive T cells in the peripheral lymphoid organs but the thymus contains cells expressing both CD4 (L3/T4) and CD8a (Ly 2). anti-mouse CD4 (L3/T4) mAb binds to approximately 85% of mouse thymocytes, 20% splenocytes, 50% lymph node cells, and a small number of bone marrow cells. It detects a protein of approximately 52 kDa on SDS-PAGE "Western Blots" (from Con A blast cell membranes) and is therefore similar to a well characterized human Leu3/ T4 antigen (4).
ncbi acc num :
CAA60883.1
ncbi gb acc num :
X87579.1
ncbi mol weight :
7,844 Da
ncbi pathways :
Adaptive Immunity Signaling Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (105986); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552)