antibody

Anti-Mouse CD3z, FITC (Clone H146-968) (hamster IgG)

MBS520101

0.1 mg

250 USD

monoclonal

hamsters

FITC

H146-968

human, mouse

immunohistochemistry, immunocytochemistry

Antibody

Anti-Mouse CD3z, FITC (Clone H146-968) (hamster IgG)

CD3z

CD3z, FITC (Clone H146-968) (hamster IgG); FITC Anti-Mouse CD3z (CD247) Monoclonal Antibody

CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3; CD247 molecule; T-cell receptor T3 zeta chain

CD3z

CD247; CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA; CD3Z; T3Z; TCRZ

CD3Z_HUMAN

Monoclonal

Hamster IgG2k

H146-968

Hamster

Mouse

164

CD3z (CD247) (Mouse CD3z (zeta chain); also known as CD247)

FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))

Antibody Concentration: 0.1 mg/ml

Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunogen: KLH conjugated mouse zeta-chain (CD3) sequence 151-164. Donor: Armenian hamster

Presentation: 100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

FITC Anti-Mouse CD3z Monoclonal Antibody detects the zeta subunit of the T cell receptor (TCR). The TCR complex consists of a ligand specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma, delta, eta and zeta subunits. This complex regulates assembly and expression of the receptor and is thought to be responsible for transmembrane transduction of signals after binding to TCRalphabeta. Studies have suggested that the CD3z subunit (also know as CD247) plays an important role in two distinct cellular compartments. In the cytoplasm, it may function to regulate Ag receptor expression and in the plasma membrane it may be required for optimal signaling by the physiologic ligand Ag/MHC. The CD3z subunit is also associated with the CD16 IgG Fc receptor on NK cells. This clone (H146-968) has been reported to work in flow cytometry (in permeabilized cells), Western blotting, and ELISA.

66841741

AAY57330.1

P20963

18,696 Da

Adaptive Immune System Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Disease Pathway (530764); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); FCGR Activation Pathway (771578); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (771577)

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. Subunit structure: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1; this interaction up-regulates the expression of the Fas ligand (FASLG) at the cell surface. Interacts with HIV-2 Nef protein; this interaction induces down-regulation of cell surface TCR/CD3 complexes. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.15 Ref.20 Ref.21 Ref.22 Ref.23. Subcellular location: Membrane; Single-pass type I membrane protein. Domain: The ITAM domains mediate interaction with SHB. Post-translational modification: Phosphorylated on Tyr residues after T-cell receptor triggering . By similarity. Involvement in disease: Immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]: An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.19. Sequence similarities: Belongs to the CD3Z/FCER1G family.Contains 3 ITAM domains.

0.1 mg

250 USD

0.3 mg

415