antibody

Anti-Rat CD25 Biotin (Clone OX-39) (mouse IgG1)

MBS520072

0.1 mg

250 USD

monoclonal

mouse

biotin

[MRC OX-39]

immunohistochemistry, flow cytometry, FACS

Antibody

Anti-Rat CD25 Biotin (Clone OX-39) (mouse IgG1)

[CD25]

[CD25 Biotin (Clone OX-39) (mouse IgG1); Biotin Anti-Rat CD25 (IL-2R) Monoclonal Antibody]

[CD25; Peripheral myelin protein 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein]

[CD25]

[Pmp22; Cd25; Pmp-22; PMP-22; SAG]

PMP22_RAT

Monoclonal

Mouse IgG1

[MRC OX-39]

Mouse

Rat

160

CD25 (IL-2Ra) (Rat CD25 (IL-2R))

Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from bioreactor supernatant))

0.1 mg/ml

Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Testing Data

Hybridoma Production: Immunization:. Immunogen:. T blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens. Donor: BALB/c spleen

FLOW CYTOMETRY ANALYSIS: Method:. 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium . 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 ?l of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 1.0-.5ug* of MBS520072 per 106 cells.5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 ul of secondary antibody (Streptavidin-PE) at a 1:50 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 ?l ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 ?l of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls).

anti-rat Interleukin-2 receptor monoclonal antibody recognizes the smaller (alpha subunit) 55kD chain of the IL-2 receptor found on activated rat T cells, thymic dendritic cells but not resting lymphocytes (1). MBS520072 binds to the rat interleukin-2 receptor and has proven to be an important marker for activated T cells (2). This clone is reported to work with frozen and paraffin sections (3).

55904

CAA44297.1

P25094

Function: Might be involved in growth regulation, and in myelinization in the peripheral nervous system. Subcellular location: Cell membrane; Multi-pass membrane protein . By similarity. Tissue specificity: Found exclusively in the peripheral nervous system. Present in both myelinating and nonmyelinating Schwann cells. Found in the tumors of Schwann cell lineage where axons are present (neurofibromas) but not where axons are absent (schwannomas). Ref.4. Developmental stage: Levels increase between embryonic day 21 (E21) and postnatal day 0 (PND0) and then gradually increase up to PND15. There is a slight increase between PND15 and adulthood. Ref.4. Induction: Strongly down-regulated in the initial phase after sciatic nerve injury. Sequence similarities: Belongs to the PMP-22/EMP/MP20 family.

0.1 mg

250 USD

0.5 mg

420