catalog number :
MBS520040
products full name :
Anti-Mouse CD11b, Purified (Clone M1/70.15) (rat IgG2b)
products short name :
CD11b
products name syn :
CD11b, Purified (Clone M1/70.15) (rat IgG2b); Purified Anti-Mouse CD11b (Mac-1; Ly 40) Monoclonal Antibody
other names :
CD11b; integrin alpha-M
products gene name :
CD11b
other gene names :
ITGAM; CD11B
specificity :
CD11b (Mac-1; Ly 40; CR3a) (Mouse CD11b (MAC-1, Ly-40))
form :
Purified: Purified IgG buffered in PBS and 0.02% NaN3 (Purified from ascitic fluid via Protein G Chromatography).
concentration :
Antibody Concentration: 1.0 mg/ml
storage stability :
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
tested application :
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Functional Assay (FA)
other info1 :
Immunogen: C57BL/6 spleen cell enriched for T lymphocytes. Donor: DA rat spleen. Fusion Partner: NS-1
other info2 :
Presentation: 250 ug purified Ig buffered in PBS and 0.02% NaN3
products description :
anti-mouse CD11b (Mac-1; Ly 40) monoclonal antibody detects the 170 kDa a subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granulocytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell mediated lysis (1,2,3,4,5,6). Applications include flow cytometry, in vitro and in vivo blocking, immunohistochemistry for both paraffin embedded and acetone-fixed frozen sections 1-20 ug/ml (#7). Also useful for immunoprecipitation and western blotting.
ncbi acc num :
AAB16869.1
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (84481); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (832066); Hematopoietic Cell Lineage Pathway (84490); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (832039); Immune System Pathway (831767); Innate Immune System Pathway (831794)