product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-Mouse CD117, FITC (Clone ACK4) (rat IgG2a)
catalog :
MBS520025
quantity :
0.1 mg
price :
220 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
ACK4
application :
flow cytometry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS520025
products type :
Antibody
products full name :
Anti-Mouse CD117, FITC (Clone ACK4) (rat IgG2a)
products short name :
CD117
products name syn :
CD117, FITC (Clone ACK4) (rat IgG2a); FITC Anti-Mouse CD117 (c-kit) Monoclonal Antibody
products gene name :
CD117
clonality :
Monoclonal
isotype :
Rat IgG2a
clone :
ACK4
host :
Rat
reactivity :
Mouse
specificity :
CD117 (c-kit) (Mouse CD117 (c-kit))
form :
FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
concentration :
Antibody Concentration: 0.1 mg/ml
storage stability :
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
tested application :
Flow Cytometry
app notes :
FLOW CYTOMETRY ANALYSIS: . 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10^7 cells/ml in media A. Add 50ul of this suspension to each tube (each tube will then contain 1x10^6 cells, representing 1 test). 4. To each tube, add 1.0ug* per cell 10^6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4 deg. C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive. 7. Wash 2 times at 4 deg. C. 8. Resuspend the cell pellet in 50ul ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media:. A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100mls). Strain Distribution by Flow Cytometry Analysis: . Cell concentration : 1x10^6 cells per test. Antibody Concentration Used: 1.0ug/10^6 cells Strain Tested: AKR, BALB/c, C3H/He, C57BL/6. Positive: AKR, BALB/c, C3H/He, C57BL/6. Negative: None
other info1 :
Immunogen: IL-3 dependent mast cells derived from WB- +/+ mice. Donor: Wistar spleen. Fusion Partner: X63.653. Ag8
other info2 :
Presentation: 100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
products description :
anti-mouse CD117 monoclonal antibody recognizes the receptor tyrosine kinase, c-kit. The ligand for this receptor is steel factor (stem cell factor), which exists in both soluble and membrane form. The interaction between steel factor and c-kit is essential for the development of hematopoietic, gonadal and pigment stem cells. c-kit positive cells are a subset of CD34+ hematopoietic precursor cells and it is expressed on 5-10% of total adult bone marrow cells. Applications: flow cytometry
size1 :
0.1 mg
price1 :
220 USD
size2 :
0.3 mg
price2 :
325
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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