protein

PDE1A, Active

MBS515627

0.005 mg

340 USD

Recombinant Protein

PDE1A, Active

[PDE1A]

[Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 1, mRNA; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; phosphodiesterase 1A; 61 kDa Cam-PDE; hCam-1]

[PDE1A]

[HCAM1; HSPDE1A; MGC26303]

[PDE1A; PDE1A; HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A; Cam-PDE 1A]

Sf9 cells

4918

Full length protein expressed in Sf9 cells

>80%

Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

0.1 ug/ul

Ships on dry ice. Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity

Puity

Specific Activity: 10,500 nmol/min/mg

Enzymes; Active Phosphodiesterases

Description: Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.

1. Lefi¨vre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67 (2):423-30. 2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98 (6):777-84.

385198066

NM_005019.4

NM_005019

P54750

~64 kDa

Ca-dependent Events Pathway (1269588); CaM Pathway (1269292); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Calmodulin Induced Events Pathway (1269293); Cam-PDE 1 Activation Pathway (1269297); DAG And IP3 Signaling Pathway (1269291); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Downstream Signal Transduction Pathway (1269479)

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]

PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase. Chromosomal Location of Human Ortholog: 2q32.1. Cellular Component: cytosol. Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity

0.005 mg

340 USD

0.01 mg

460