protein

PDE1C, Active

MBS515626

0.005 mg

340 USD

Recombinant Protein

PDE1C, Active

[PDE1C]

[Homo sapiens phosphodiesterase 1C (PDE1C), transcript variant 4, mRNA; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; phosphodiesterase 1C; hCam-3]

[PDE1C]

[Hcam3]

[PDE1C; PDE1C; Hcam3; hCam-3; cam-PDE 1C; Cam-PDE 1C]

Sf9 cells

Full length recombinant protein expressed in Sf9 cells

>90%

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

0.1 ug/ul

Shipping: Dry Ice. Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Specific Activity: 445 nmol/min/mg

Enzymes; Active Phosphodiesterases

Description: Full-length recombinant human PDE1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: PDE1C is a member of the phosphodiesterases (PDE) family which catalyzes the hydrolysis of cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C binds both cAMP and cGMP with high affinity and hydrolyzes both substrates with similar rates of catalysis (1). PDE1C is a calmodulin-dependent PDE and is stimulated by calcium-calmodulin complex. PDE1C is expressed at high levels in human cardiac myocytes with an intracellular distribution distinct from that of other phosphodiesterases (2). PDE1C levels decrease in all conditions that inhibited cell proliferation and PDE1C is a useful marker in studying the dynamics of proliferation and migration of various cells.

1. Loughney, K. et al: Isolation and characterization of cDNAs corresponding to two human calcium, calmodulin-regulated, 3-prime, 5-prime-cyclic nucleotide phosphodiesterases. J. Biol. Chem. 271: 796-806, 1996. 2. Vandeput, F. et al; Cyclic nucleotide phosphodiesterase PDE1C1 in human cardiac myocytes. J. Biol. Chem. 282: 32749-42757, 2007.

1015120667

NM_005020.4

NM_005020

Q14123

~99 kDa

Ca-dependent Events Pathway (1269588); CaM Pathway (1269292); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Calmodulin Induced Events Pathway (1269293); Cam-PDE 1 Activation Pathway (1269297); DAG And IP3 Signaling Pathway (1269291); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Downstream Signal Transduction Pathway (1269479)

This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]

PDE1C: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase. Chromosomal Location of Human Ortholog: 7p14.3. Cellular Component: cytosol. Molecular Function: calmodulin-dependent cyclic-nucleotide phosphodiesterase activity

0.005 mg

340 USD

0.01 mg

460