protein

PDE1B, Active

MBS515625

0.005 mg

340 USD

Recombinant Protein

PDE1B, Active

[PDE1B]

[Homo sapiens phosphodiesterase 1B (PDE1B), transcript variant 1, mRNA; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; phosphodiesterase 1B; 63 kDa Cam-PDE]

[PDE1B]

[PDE1B1; PDES1B]

[PDE1B; PDE1B; PDE1B1; PDES1B; HEL-S-79p; PDE1B1; PDES1B; Cam-PDE 1B]

Sf9 cells

>90%

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

0.1 ug/ul

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: Ships with dry ice

Purity

Specific Activity: 100 nmol/min/mg

Enzymes; Active Phosphodiesterases

Description: Full-length recombinant human PDE1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: PDE1B is a member of the PDE protein superfamily that catalyzes the hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1B is a calmodulin-dependent enzyme that is stimulated by calcium-calmodulin complex (1). PDE1B is express in the lymphoblastic and leukemic cell lines and causes the cells to undergo apoptosis, suggesting PDE1B could be a novel therapeutic strategy for the treatment of leukemia (2). GM-CSF is a major regulator of monocyte to macrophage differentiation and this cytokine has been shown to up-regulate PDE1B.

1. Yu, J.et.al: Identification and characterisation of a human calmodulin-stimulated phosphodiesterase PDE1B1. Cell. Signal. 9: 519-529, 1997. 2. Jiang, X. et.al: Inhibition of calmodulin-dependent phosphodiesterase induces apoptosis in human leukemic cells. Proc. Nat. Acad. Sci. 93: 11236-11241, 1996.

260436978

NM_000924.3

NM_000924

Q01064

~86 kDa

Ca-dependent Events Pathway (1269588); CaM Pathway (1269292); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Calmodulin Induced Events Pathway (1269293); Cam-PDE 1 Activation Pathway (1269297); DAG And IP3 Signaling Pathway (1269291); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Downstream Signal Transduction Pathway (1269479)

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: cytosol; nucleus. Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; protein binding. Biological Process: apoptosis; cAMP catabolic process; cGMP catabolic process; monocyte differentiation

0.005 mg

340 USD

0.01 mg

460