MLL2 (KMT2D), Active | MyBioSource MBS515547 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MLL2 (KMT2D), Active

catalog :

MBS515547

quantity :

0.005 mg

price :

340 USD

more info or order :

MyBioSource product webpage

image

image 1 :

The specific activity of MLL2 (KMT2D) was determined to be 2.5 nmol/min/mg as per activity assay protocol.

image 2 :

The purity of MLL2 (KMT2D) was determined to be >80% by densitometry, approx. MW 51kDa

product information

catalog number :

MBS515547

products type :

Recombinant Protein

products full name :

MLL2 (KMT2D), Active

products short name :

[MLL2]

other names :

[Homo sapiens lysine methyltransferase 2D (KMT2D), mRNA; Histone-lysine N-methyltransferase 2D; histone-lysine N-methyltransferase 2D; lysine methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2]

products gene name :

[MLL2]

products gene name syn :

[MLL2; MLL4; KMT2D; AAD10; ALR; CAGL114; KABUK1; KMS; TNRC21]

other gene names :

[KMT2D; KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114; ALR; MLL2; MLL4; Lysine N-methyltransferase 2D]

host :

Sf9 insect cells using baculovirus

sequence positions :

[5317-end]

sequence length :

19432

purity :

>80%

form :

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

concentration :

0.05 mg/ml

storage stability :

Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: Ships with dry ice. Stability: 1 year at -70°C from date of shipment.

tested application :

Methyltransferase Assay

image1 heading :

Testing Data

image2 heading :

Testing Data #2

other info1 :

Species: Human. Spcific Activity: 2.5 nmol/min/mg

other info2 :

Tag: GST tag

products categories :

Enzymes; Active Methyltransferases

products description :

Description: Recombinant human MLL2 (5317-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: Histone-lysine N-methyltransferase 2D, also known as mixed-lineage leukemia 2 (MLL2), methylates 'Lys-4' of histone H3 (H3K4me). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. MLL2/KMT2D is among the most frequently mutated genes in a variety of pediatric tumors.

products references :

1. Rao RC, et al: Hijacked in cancer: the KMT2 (MLL) family of methyltransferases. Nat Rev Cancer. 15 (6):334-46. 2015. 2. Huether R. et al: The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomes. Nat Commun 5: 3630. 2014. http://www.uniprot.org/uniprot/O14686

ncbi gi num :

148762968

ncbi acc num :

NM_003482.3

ncbi gb acc num :

NM_003482

uniprot acc num :

O14686

ncbi mol weight :

51 kDa

ncbi pathways :

Activation Of HOX Genes During Differentiation Pathway (1339139); Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway (1339140); Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Deactivation Of The Beta-catenin Transactivating Complex Pathway (1269604); Developmental Biology Pathway (1270302); Formation Of The Beta-catenin:TCF Transactivating Complex Pathway (1269602); Histone Modifications Pathway (920986); Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320)

ncbi summary :

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

uniprot summary :

MLL4: Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Nuclear receptor co-regulator; Transcription regulation. Chromosomal Location of Human Ortholog: 12q13.12. Cellular Component: histone methyltransferase complex; nucleoplasm. Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding. Biological Process: chromatin silencing; histone H3-K4 methylation; oocyte growth; oogenesis; positive regulation of cell proliferation; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; response to estrogen stimulus. Disease: Kabuki Syndrome 1

size1 :

0.005 mg

price1 :

340 USD

size2 :

0.01 mg

price2 :

460

more info or order :

MyBioSource product webpage