product summary
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company name :
MyBioSource
product type :
protein
product name :
ERF1 Protein
catalog :
MBS515495
quantity :
0.02 mg
price :
345 USD
more info or order :
image
image 1 :
The purity of ERF1 was determined to be >95% by densitometry, approx. MW 74 kDa
product information
catalog number :
MBS515495
products type :
Recombinant Protein
products full name :
ERF1 Protein
products short name :
[ERF1]
other names :
[Homo sapiens eukaryotic translation termination factor 1 (ETF1), transcript variant 1, mRNA; Eukaryotic peptide chain release factor subunit 1; eukaryotic peptide chain release factor subunit 1; eukaryotic translation termination factor 1; Protein Cl1; TB3-1]
products gene name :
[ERF1]
products gene name syn :
[eRF1; ETF1; D5S1995; ERF; RF1; SUP45L1; TB3-1]
other gene names :
[ETF1; ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1; ERF1; RF1; SUP45L1; Eukaryotic release factor 1; eRF1]
host :
Sf9 insect cells using baculovirus
sequence positions :
[2-end]
sequence length :
3748
purity :
>95%
form :
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
concentration :
0.1ug/ul
storage stability :
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: Dry Ice.
tested application :
Enzyme Assay, Western Blot (WB)
image1 heading :
Testing Data
other info1 :
Species: Human
products categories :
Signaling Proteins; Cellular Proteins
products description :
Description: Recombinant human ERF1 (2-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: Eukaryotic peptide chain release factor subunit 1 (eRF1) directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
products references :
1. Zilka, N., et al. Truncated tau from sporadic Alzheimer's disease suffices to drive neurofibrillary degeneration in vivo. FEBS Lett. 2006; 508: 3582-3588. 2. Rial, A. et al: Calcium Dyshomeostasis in beta-Amyloid and Tau-bearing Skeletal Myotubes. J. Biol. Chem., 2004; 279: 3524-53532
ncbi gi num :
372626411
ncbi acc num :
NM_004730.3
ncbi gb acc num :
NM_004730
uniprot acc num :
P62495
ncbi mol weight :
74kDa
ncbi pathways :
Eukaryotic Translation Termination Pathway (1268692); Gene Expression Pathway (1269649); Metabolism Of Proteins Pathway (1268677); Nonsense Mediated Decay (NMD) Enhanced By The Exon Junction Complex (EJC) Pathway (1269717); Nonsense Mediated Decay (NMD) Independent Of The Exon Junction Complex (EJC) Pathway (1269718); Nonsense-Mediated Decay (NMD) Pathway (1269716); Translation Pathway (1268678); Translation Factors Pathway (198803); MRNA Surveillance Pathway (193328); MRNA Surveillance Pathway (193184)
ncbi summary :
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013]
uniprot summary :
ETF1: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Belongs to the eukaryotic release factor 1 family. Chromosomal Location of Human Ortholog: 5q31.2. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: protein binding; ribosome binding; RNA binding; translation release factor activity; translation termination factor activity. Biological Process: mRNA catabolic process, nonsense-mediated decay; protein amino acid methylation; regulation of translational termination; translational termination
size1 :
0.02 mg
price1 :
345 USD
size2 :
0.05 mg
price2 :
540
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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