TPX2 recombinant protein | MyBioSource MBS515412 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

TPX2 recombinant protein

catalog :

MBS515412

quantity :

0.02 mg

price :

235 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS515412

products type :

Recombinant Protein

products full name :

TPX2 recombinant protein

products short name :

[TPX2]

products name syn :

[DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1]

other names :

[Homo sapiens TPX2, microtubule-associated (TPX2), mRNA; Targeting protein for Xklp2; targeting protein for Xklp2; protein fls353; TPX2, microtubule-associated, homolog; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2; TPX2, microtubule-associated; Differentially expressed in cancerous and non-cancerous lung cells 2; DIL-2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression proliferation-associated protein 100]

products gene name :

[TPX2]

products gene name syn :

[DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1]

other gene names :

[TPX2; TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1; C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100]

uniprot entry name :

TPX2_HUMAN

host :

Sf9 insect cells

sequence length :

3685

purity :

>80%

form :

50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

concentration :

0.2ug/ul

storage stability :

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantitites after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on Dry Ice.

tested application :

Kinase Assay, Western Blot (WB)

image1 heading :

SDS-PAGE

other info1 :

Type: Recombinant Fusion Protein. Species: Human. Tag Information: His tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant full-length human TPX2 protein was expressed by baculovirus in Sf9 insect cells. This product can be used for Aurora A activation in vitro.

products categories :

Microtubule/Actin Associated Proteins; Signaling Proteins - Microtubule/Actin Associated Proteins

products description :

Recombinant full-length human TPX2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. This product can be used for Aurora A activation in vitro. Scientific Background: TPX2 is a nuclear proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M (1). It mainly expressed in the lung carcinoma cell lines and also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney.TPX2 is required for targeting STK6 to the spindle apparatus, and that STK6 may regulate the function of TPX2 during spindle assembly. TPX2 is mailnly function in mitotic spindle assembly likely contributes to its role in chromosome stability control and tumor suppression (2).

products references :

1. Heidebrecht, H. J. et.al: p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M. Blood 90: 226-233, 1997. 2. Joukov, V.et.al: The BRCA1/BARD1 heterodimer modulates Ran-dependent mitotic spindle assembly. Cell 127: 539-552, 2006.

ncbi gi num :

40354199

ncbi acc num :

NP_036244.2

ncbi gb acc num :

NM_012112

uniprot acc num :

Q9ULW0

ncbi mol weight :

~110 kDa

ncbi pathways :

Aurora A Signaling Pathway (137925); Gastric Cancer Network 1 Pathway (760635); PLK1 Signaling Events Pathway (138007)

uniprot summary :

Function: Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. Ref.13 Ref.17. Subunit structure: Interacts with AURKA. Ref.13 Ref.22 Ref.23. Subcellular location: Nucleus. Cytoplasm cytoskeleton spindle. Cytoplasm cytoskeleton spindle pole. Note: During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. Ref.13 Ref.17. Tissue specificity: Expressed in lung carcinoma cell lines but not in normal lung tissues. Developmental stage: Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. Sequence similarities: Belongs to the TPX2 family.

size1 :

0.02 mg

price1 :

235 USD

size2 :

0.05 mg

price2 :

365

more info or order :

MyBioSource product webpage