catalog number :
MBS515401
products type :
Recombinant Protein
products full name :
GST recombinant protein
products short name :
[GST]
other names :
[pGEX-2T cloning vector, complete sequence]
products gene name :
[GST]
form :
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM Glutathione, 0.1 mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
concentration :
0.2 ug/ul
storage stability :
Stability:. 1 yr At -70°C from date of shipment. Storage & Shipping:. Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: DRY ICE.
tested application :
Western Blot (WB)
image1 heading :
SDS-PAGE
products categories :
Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins
products description :
Description: . Recombinant artificial sequences GST (1-226) was expressed in E. coli cells and purified on glutathione column. Scientific Background: . GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called GST gene fusion system (1). These proteins are capable of multiple reactions with a multitude of substrates. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione (1).
products references :
1. Beckett, G.J. and Hayes, J.D. (1987). Glutathione S-transferase measurements and liver disease in man. J. Clin. Biochem. Nutr. 2, 1-24. .