protein

14-3-3 alpha/beta recombinant protein

MBS515396

0.02 mg

225 USD

Recombinant Protein

14-3-3 alpha/beta recombinant protein

14-3-3 alpha/beta

14-3-3 alpha/beta; YWHAB; HS1; GW128; KCIP-1; 14-3-3alpha/beta Protein; 14-3-3a/b Protein

Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 1, mRNA; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; Protein 1054; Protein kinase C inhibitor protein 1

14-3-3 alpha/beta

14-3-3 alpha/beta; YWHAB; HS1; GW128; KCIP-1

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; KCIP-1

1433B_HUMAN

E Coli

3231

50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

Type: Recombinant Fusion Protein. Species: Human. Tag Information: tag-free. Expression System: E.coli. Source Note: Recombinant full-length human tag-free 14-3-3alpha/beta was expressed in E. coli cells

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

Recombinant full-length human tag-free 14-3-3alpha/beta was expressed in E. coli cells. Scientific Background: 14-3-3alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.

1.Yaffe, M B. et al: The structural basis for 14-3-3:phosphopeptide binding specificity. Cell 91: 961-971, 1997. 2.Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.

520975501

NP_003395.1

NM_003404

P31946

~29kDa

ARMS-mediated Activation Pathway (106466); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Axon Guidance Pathway (105688); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Ref.13 Ref.15. Subunit structure: Homodimer. Interacts with SAMSN1 and PRKCE . By similarity. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2 and YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.19. Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.11. Post-translational modification: The alpha, brain-specific form differs from the beta form in being phosphorylated . By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion . By similarity. Ref.13Isoform Short contains a N-acetylmethionine at position 1 . By similarity. Sequence similarities: Belongs to the 14-3-3 family.

0.02 mg

225 USD

0.05 mg

340