UBLE1A (SAE1) recombinant protein | MyBioSource MBS515376 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

UBLE1A (SAE1) recombinant protein

catalog :

MBS515376

quantity :

0.02 mg

price :

260 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS515376

products type :

Recombinant Protein

products full name :

UBLE1A (SAE1) recombinant protein

products short name :

UBLE1A (SAE1)

products name syn :

SAE1; SUA1; HSPC140; AOS1; FLJ3091; HSPC140; UBLE1A(SAE1) protein

other names :

Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mRNA; SUMO-activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUMO1 activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A

products gene name :

UBLE1A (SAE1)

products gene name syn :

SAE1; SUA1; HSPC140; AOS1; FLJ3091; HSPC140

other gene names :

SAE1; SAE1; AOS1; SUA1; UBLE1A; HSPC140; AOS1; SUA1; UBLE1A

uniprot entry name :

SAE1_HUMAN

host :

Sf9 insect cells

sequence length :

2538

form :

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

storage stability :

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

tested application :

Western Blot (WB)

other info1 :

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant full-length human UBLE1A (SAE1) was expressed by baculovirus in Sf9 insect cells

products categories :

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

products description :

Recombinant full-length human UBLE1A (SAE1) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: UBLE1A (also known as SAE1) or SUMO1 activating enzyme subunit 1 is involved in regulating protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (1). The SAE1/SAE2 dimer functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes. The SAE2 inactivation may be a therapeutic strategy in MYC-driven cancers (2).

products references :

1. Okuma, T. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999. 2. Kessler, J. D. et.al: A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 335: 348-353, 2012.

ncbi gi num :

225543279

ncbi acc num :

NP_005491.1

ncbi gb acc num :

NM_005500

uniprot acc num :

Q9UBE0

ncbi mol weight :

~65 kDa

ncbi pathways :

Conjugation Of SUMO To E1 (UBA2:SAE1) Pathway (833808); Processing And Activation Of SUMO Pathway (833806); SUMOylation Pathway (833805); Transfer Of SUMO From E1 To E2 (UBE2I, UBC9) Pathway (833809); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

uniprot summary :

Function: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Ref.1 Ref.2 Ref.3 Ref.11 Ref.12 Ref.16 Ref.17. Pathway: Protein modification; protein sumoylation. Subunit structure: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. Ref.1 Ref.2 Ref.3 Ref.11. Subcellular location: Nucleus Ref.11. Tissue specificity: Expression level increases during S phase and drops in G2 phase (at protein level). Ref.11. Sequence similarities: Belongs to the ubiquitin-activating E1 family.

size1 :

0.02 mg

price1 :

260 USD

size2 :

0.05 mg

price2 :

415

more info or order :

MyBioSource product webpage