MDM4 (1-137) recombinant protein | MyBioSource MBS515348 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MDM4 (1-137) recombinant protein

catalog :

MBS515348

quantity :

0.02 mg

price :

260 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS515348

products type :

Recombinant Protein

products full name :

MDM4 (1-137) recombinant protein

products short name :

MDM4

products name syn :

DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4(1-137) Protein

other names :

Homo sapiens Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1, mRNA; Protein Mdm4; protein Mdm4; protein Mdmx; MDM4-related protein 1; mdm2-like p53-binding protein; double minute 4, human homolog of; p53-binding protein; Mdm4 p53 binding protein homolog (mouse); Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4

products gene name :

MDM4

products gene name syn :

DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1

other gene names :

MDM4; MDM4; HDMX; MDMX; MRP1; MDMX

uniprot entry name :

MDM4_HUMAN

host :

E Coli

sequence positions :

1-137

sequence length :

10090

purity :

>75%

form :

50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

concentration :

0.2 ug/ul

storage stability :

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. STable for 1 year at -70 degree C from date of shipment.

tested application :

Western Blot (WB)

other info1 :

Type: Recombinant Fusion Protein. Species: Human. Tag Information: His tag. Expression System: E.coli. Source Note: Recombinant human MDM4 (1-137) was expressed in E. coli cells

products categories :

Cell Cycle Proteins; Signaling Proteins - Cell Cycle Proteins

products description :

Recombinant human MDM4 (1-137) was expressed in E. coli cells using an N-terminal His tag. Scientific Background: MDM4 is a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus. MDM4 shows structural similarity to p53-binding protein MDM2 and both proteins bind the p53 tumor suppressor protein and inhibit its activity. However, unlike MDM2, MDM4 does not cause nuclear export or degradation of p53. Instead, MDM4 inhibits p53 activity by binding to the transcriptional activation domain of p53. MDM4 is overexpressed in a variety of human cancers (1). Expression level of MDM4 is significantly higher in chronic lymphocytic leukemia. MDM4 is a specific chemotherapeutic target for treating retinoblastoma (2).

products references :

1. Parant, J.et.al: Rescue of embryonic lethality in Mdm4-null mice by loss of Trp53 suggests a nonoverlapping pathway with MDM2 to regulate p53. Nature Genet. 29: 92-95, 2001. 2. Laurie, N. A. et.al: Inactivation of the p53 pathway in retinoblastoma. Nature 444: 61-66, 2006.

ncbi gi num :

323510631

ncbi acc num :

NP_002384.2

ncbi gb acc num :

NM_002393

uniprot acc num :

O15151

ncbi mol weight :

~17.3 kDa

ncbi pathways :

MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Signaling Pathways In Glioblastoma (672458); P53 Pathway (138067); P53 Signaling Pathway (83055); P53 Signaling Pathway (465)

ncbi summary :

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

uniprot summary :

Function: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Ref.9 Ref.10. Subunit structure: Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Ref.9 Ref.10 Ref.11. Subcellular location: Nucleus. Tissue specificity: Expressed in all tissues tested with high levels in thymus. Induction: Down-regulated by cisplatin (at protein level). Ref.11. Domain: Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2. Post-translational modification: Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent. Ref.9 Ref.10Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein. Ref.9 Ref.11. Sequence similarities: Belongs to the MDM2/MDM4 family.Contains 1 RanBP2-type zinc finger.Contains 1 RING-type zinc finger.Contains 1 SWIB domain. Mass spectrometry: Molecular mass is 54863.3 Da from positions 1 - 490. Determined by MALDI. Ref.12

size1 :

0.02 mg

price1 :

260 USD

size2 :

0.05 mg

price2 :

415

more info or order :

MyBioSource product webpage