protein

STAT5 recombinant protein

MBS515346

0.02 mg

260 USD

Recombinant Protein

STAT5 recombinant protein

STAT5

MGF; STAT5A

Homo sapiens signal transducer and activator of transcription 5A (STAT5A), mRNA; Signal transducer and activator of transcription 5A; signal transducer and activator of transcription 5A; signal transducer and activator of transcription 5A

STAT5

MGF; STAT5A

STAT5A; STAT5A; MGF; STAT5; STAT5

STA5A_HUMAN

Sf9 insect cells

4314

>90%

Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.

0.2ug/ul

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Kinase Assay, Western Blot (WB)

Type: Recombinant Fusion Protein. Species: Human. Tag Information: His tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant full-length human STAT5 was expressed by baculovirus in Sf9 insect cells

Acession Number: NM 0031512

Transcription Proteins; Signaling Proteins - Transcription Proteins

Recombinant full-length human STAT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. Scientific Background: STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).

1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996. 2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173 -2178.

221316717

NP_003143.2

NM_003152

P42229

~92 kDa

AGE/RAGE Pathway (698754); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198832); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); BDNF Signaling Pathway (712093); CD40/CD40L Signaling Pathway (138061); CXCR4-mediated Signaling Events Pathway (137910); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528)

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]

Function: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. Ref.7 Ref.12. Subunit structure: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 . By similarity. Interacts with NCOA1 and SOCS7. Interacts with ERBB4. Ref.8 Ref.12 Ref.13. Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation. Ref.12. Post-translational modification: Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity . By similarity. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. Ref.7 Ref.9 Ref.10 Ref.17ISGylated . By similarity. Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.

0.02 mg

260 USD

0.05 mg

415