product summary
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company name :
MyBioSource
product type :
protein
product name :
PFKFB4 recombinant protein
catalog :
MBS515316
quantity :
0.02 mg
price :
260 USD
more info or order :
product information
catalog number :
MBS515316
products type :
Recombinant Protein
products full name :
PFKFB4 recombinant protein
products short name :
PFKFB4
products name syn :
PFKFB4 protein
other names :
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), mRNA; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4; bifunctional enzyme with kinase and biphosphatase activities; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme
products gene name :
PFKFB4
other gene names :
PFKFB4; PFKFB4; 6PF-2-K/Fru-2,6-P2ase 4
uniprot entry name :
F264_HUMAN
host :
Sf9 insect cells
sequence length :
3503
purity :
>90%
form :
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
concentration :
0.1ug/ul
storage stability :
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
tested application :
Western Blot (WB)
other info1 :
Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant human PFKFB4 (2-end) was expressed by baculovirus in Sf9 insect cells
other info2 :
Acession Number: NM 004567
products categories :
Fructose Kinases Proteins; Signaling Proteins - Fructose Kinase Proteins
products description :
Recombinant human PFKFB4 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: PFKFB4 (also known as 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4) regulates the steady-state concentration of fructose-2, 6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis pathway called phosphofructokinase. Isozymes of PFKFB differ in the regions surrounding the catalytic core, which are important for the differential response to allosteric effectors and hormonal signals in different tissues (1). PFKFB4 is highly expressed in brain, heart, liver, muscle, placenta, adipose tissue, ovary, fallopian tubes, and testis (2).
products references :
1. Sakai, A. et.al: Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate,2-kinase/fructose 2,6-bisphosphatase from human placenta. J. Biochem. 119: 506-511, 1996. 2. Manzano, A.et.al: Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene. Gene 229: 83-89, 1999.
ncbi gi num :
19923257
ncbi acc num :
NP_004558.1
ncbi gb acc num :
NM_004567
uniprot acc num :
Q16877
ncbi mol weight :
~82 kDa
ncbi pathways :
Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Glucose Metabolism Pathway (106199); Glycolysis Pathway (105911); HIF-1 Signaling Pathway (695200); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)
uniprot summary :
Function: Synthesis and degradation of fructose 2,6-bisphosphate. Catalytic activity: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate. Enzyme regulation: The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme . By similarity. Subunit structure: Homodimer . By similarity. Sequence similarities: In the C-terminal section; belongs to the phosphoglycerate mutase family.
size1 :
0.02 mg
price1 :
260 USD
size2 :
0.05 mg
price2 :
415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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