LOXL1 recombinant protein | MyBioSource MBS515307 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

LOXL1 recombinant protein

catalog :

MBS515307

quantity :

0.02 mg

price :

260 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS515307

products type :

Recombinant Protein

products full name :

LOXL1 recombinant protein

products short name :

LOXL1

products name syn :

LOL; LOXL; LOXL1 Protein

other names :

Homo sapiens lysyl oxidase-like 1, mRNA; Lysyl oxidase homolog 1; lysyl oxidase homolog 1; lysyl oxidase-like protein 1; lysyl oxidase-like 1; Lysyl oxidase-like protein 1

products gene name :

LOXL1

products gene name syn :

LOL; LOXL

other gene names :

LOXL1; LOXL1; LOL; LOXL; LOXL; LOL

uniprot entry name :

LOXL1_HUMAN

host :

Sf9 insect cells

sequence length :

2346

purity :

>70%

form :

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 50mM NaCl and 25% glycerol.

concentration :

0.1ug/ul

storage stability :

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

tested application :

Western Blot (WB)

other info1 :

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant human LOXL1 (292-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

other info2 :

Gene Accession Number: BC015090

products categories :

Lysyl Oxidase Proteins; Signaling Proteins - Lysyl Oxidase Proteins

products description :

Recombinant human LOXL1 (292-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: LOXL1 is a member of the lysyl oxidase gene family which is essential to the biogenesis of connective tissue. LOXL1 encodes an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. LOXL1 is responsible for catalyzing the oxidative deamination of lysine residues of tropoelastin and this deamination causes spontaneous cross-linking and formation of elastin polymer fibers (1). LOXL1 serves both as a crosslinking enzyme and an element of the scaffold to ensure spatially defined deposition of elastin (2).

products references :

1. Hewitt, A. W.et.al: Ancestral LOXL1 variants are associated with pseudoexfoliation in Caucasian Australians but with markedly lower penetrance than in Nordic people. Hum. Molec. Genet. 17: 710-716, 2008. 2. Liu, X. et.al: Elastic fiber homeostasis requires lysyl oxidase-like 1 protein. Nature Genet. 36: 178-182, 2004.

ncbi gi num :

33878694

ncbi acc num :

BC015090

uniprot acc num :

Q08397

ncbi mol weight :

~61 kDa

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Active on elastin and collagen substrates . By similarity. Cofactor: Copper . By similarity.Contains 1 lysine tyrosylquinone . By similarity. Subcellular location: Secreted extracellular space . Potential. Tissue specificity: Expressed in ocular tissues including the iris, ciliary body, lens and optic nerve. Not detected in the retina. Ref.7. Post-translational modification: The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Involvement in disease: Exfoliation syndrome (XFS) [MIM:177650]: A disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Ref.6. Sequence similarities: Belongs to the lysyl oxidase family.

size1 :

0.02 mg

price1 :

260 USD

size2 :

0.05 mg

price2 :

415

more info or order :

MyBioSource product webpage