protein

VAV1 recombinant protein

MBS515284

0.02 mg

330 USD

Recombinant Protein

VAV1 recombinant protein

VAV1

VAV

Homo sapiens vav 1 guanine nucleotide exchange factor (VAV1), transcript variant 1, mRNA; Proto-oncogene vav; proto-oncogene vav; vav 1 oncogene; vav 1 guanine nucleotide exchange factor

VAV1

VAV

VAV1; VAV1; VAV; VAV

VAV_HUMAN

Sf9 insect cells

2944

The purity of VAV1 was determined to be > 70% by densitometry.

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant human VAV1 (73-end) was expressed by baculovirus in Sf9 insect cells

Cellular Proteins; Signaling Proteins - Cellular Proteins

Recombinant human VAV1 (73-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: VAV1 is a member of the VAV gene family which are guanine nucleotide exchange factors (GEFs) for Rho family GTPases. VAV1 activates pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. VAV1 is important in hematopoiesis, playing a role in T-cell and B-cell development and activation (1). VAV1 has been identified as the specific binding partner of Nef proteins from HIV-1. Co-expression and binding of Nef with VAV1 initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.VAV1 proto-oncogene participates in the signaling processes that mediate the antigen-induced activation of B lymphocytes (2).

1. Zhang, R. et.al:. Defective signalling through the T- and B-cell antigen receptors in lymphoid cells lacking the vav proto-oncogene. Nature 374: 470-473, 1995. 2. Bustelo, X. R.et.al: Tyrosine phosphorylation of the VAV proto-oncogene product in activated B cells. Science 256: 1196-1199, 1992.

384551644

NP_005419.2

NM_005428

P15498

~120 kDa

Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); CD28 Co-stimulation Pathway (119553); CD28 Dependent Vav1 Pathway (119555); CXCR4-mediated Signaling Events Pathway (137910); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Chemokine Signaling Pathway (99051)

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. Subunit structure: May interact with CCPG1 . By similarity. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK . By similarity. Interacts with THEMIS2. Interacts with HCK . By similarity. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54 . By similarity. Ref.10 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.21 Ref.22. Tissue specificity: Widely expressed in hematopoietic cells but not in other cell types. Domain: The DH domain is involved in interaction with CCPG1 . By similarity. Post-translational modification: Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS) . By similarity. Phosphorylated by FYN. Ref.14. Miscellaneous: 'Vav' stands for the sixth letter of the Hebrew alphabet. Sequence similarities: Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains. Sequence caution: The sequence BAG62721.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA34383.1 differs from that shown. Reason: Frameshift at positions 322 and 355.

0.02 mg

330 USD

0.05 mg

500