STK9 recombinant protein | MyBioSource MBS515242 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

STK9 recombinant protein

catalog :

MBS515242

quantity :

0.02 mg

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

The purity of STK9 was determined to be >85% by densitometry, approx MW 84 kDa.

product information

catalog number :

MBS515242

products type :

Recombinant Protein

products full name :

STK9 recombinant protein

products short name :

[STK9]

products name syn :

[CDKL5; EIEE2; ISSX]

other names :

[Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), transcript variant I, mRNA; Cyclin-dependent kinase-like 5; cyclin-dependent kinase-like 5; serine/threonine kinase 9; serine/threonine-protein kinase 9; cyclin dependent kinase 5 transcript; cyclin-dependent kinase-like 5; Serine/threonine-protein kinase 9]

products gene name :

[STK9]

products gene name syn :

[CDKL5; EIEE2; ISSX]

other gene names :

[CDKL5; CDKL5; ISSX; STK9; EIEE2; STK9]

uniprot entry name :

CDKL5_HUMAN

host :

Sf9 insect cells

sequence positions :

[1-498]

sequence length :

3434

purity :

>85%

form :

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

concentration :

0.1 ug/ uL

storage stability :

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Ships with Dry Ice.

tested application :

Western Blot (WB)

image1 heading :

SDS-PAGE

other info1 :

Source Note: Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells

products categories :

Cellular Proteins; Signaling Proteins - Cellular Proteins

products description :

Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).

products references :

1. Ricciardi, S. et.al: CDKL5 influences RNA splicing activity by its association to the nuclear speckle molecular machinery. Hum. Molec. Genet. 18: 4590-4602, 2009. 2. Archer, H. L. et.al: CDKL5 mutations cause infantile spasms, early onset seizures, and severe mental retardation in female patients. J. Med. Genet. 43: 729-734, 2006.

ncbi gi num :

83367066

ncbi acc num :

NP_003150.1

ncbi gb acc num :

NM_003159

uniprot acc num :

O76039

ncbi mol weight :

~84 kDa

ncbi pathways :

BDNF Signaling Pathway (712093)

ncbi summary :

This gene is a member of Ser/Thr protein kinase family and encodes a phosphorylated protein with protein kinase activity. Mutations in this gene have been associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Mediates phosphorylation of MECP2. Ref.7 Ref.8. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Subunit structure: Interacts with MECP2. Ref.7. Subcellular location: Nucleus Ref.8. Tissue specificity: Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis. Post-translational modification: Autophosphorylated. Ref.7 Ref.8. Involvement in disease: Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).Epileptic encephalopathy, early infantile, 2 (EIEE2) [MIM:300672]: A severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients with epileptic encephalopathy early infantile type 2 manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. However, EIEE2 and Rett syndrome are considered two distinct entities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.6 Ref.7 Ref.8 Ref.13 Ref.14 Ref.15 Ref.16 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain. Caution: It is uncertain whether Met-1 or Met-10 is the initiator. Sequence caution: The sequence CAA61445.1 differs from that shown. Reason: Frameshift at position 415.

size1 :

0.02 mg

price1 :

270 USD

size2 :

0.05 mg

price2 :

450

more info or order :

MyBioSource product webpage