protein

KDM1A recombinant protein

MBS515219

0.02 mg

260 USD

Recombinant Protein

KDM1A recombinant protein

KDM1A

AOF2; BHC110; LSD1; KIAA0601

Homo sapiens amine oxidase (flavin containing) domain 2, mRNA; Lysine-specific histone demethylase 1A; lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2

KDM1A

AOF2; BHC110; LSD1; KIAA0601

KDM1A; KDM1A; AOF2; KDM1; LSD1; BHC110; AOF2; KDM1; KIAA0601; LSD1

KDM1A_HUMAN

Sf9 insect cells

2969

>95%

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

0.2 ug/ul

Store product 1yr at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant human KDM1A (172-end) was expressed by baculovirus in Sf9 insect cells. Gene Acession Number: BC048134

Deacetylases/Demethylase Proteins; Signaling Proteins - Deacetylase/Demethylase Proteins

Recombinant human KDM1A (172-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: KDM1A or lysine (K)-specific demethylase 1A is a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain that is a component of several histone deacetylase complexes and act as a histone demethylase. (1) H3K4 histone demethylase activity of KDM1A is partly responsible for the repressive activity of TAL1 and restricts TAL1 function in hematopoiesis (1). KDM1A plays an essential role for CoREST in demethylation of H3K4 both in vitro and in vivo (2).

1. Hu, X. et.al : LSD1-mediated epigenetic modification is required for TAL1 function and hematopoiesis. Proc. Nat. Acad. Sci. 106: 10141-10146, 2009. 2. Lee, M. G. et.al : An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation. Nature 437: 432-435, 2005.

28856184

BC048134

O60341

~100 kDa

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Notch Signaling Pathway (169345); Notch-mediated HES/HEY Network Pathway (169347)

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Function: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. Ref.5 Ref.8 Ref.12 Ref.15 Ref.23 Ref.24. Cofactor: FAD. Ref.8 Ref.9. Subunit structure: Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B . By similarity. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Interacts with SNAI1 (via SNAG domain). Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.22 Ref.24. Subcellular location: Nucleus Ref.6 Ref.12. Tissue specificity: Ubiquitously expressed. Ref.12. Domain: The SWIRM domain may act as an anchor site for a histone tail. Ref.30. Sequence similarities: Belongs to the flavin monoamine oxidase family.Contains 1 SWIRM domain. Biophysicochemical propertiesKinetic parameters:KM=3.0 uM for H3 monomethyl-K4 Ref.13KM=4.2 uM for H3 dimethyl-K4KM=3.9 uM for H3 monomethyl-K4-monomethyl-K9KM=17.5 uM for monomethyl-K4-acetyl-K9. Sequence caution: The sequence BAA25527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.02 mg

260 USD

0.05 mg

415