protein

TET1 recombinant protein

MBS515125

0.02 mg

270 USD

Recombinant Protein

TET1 recombinant protein

[TET1]

[bA119F7.1; CXXC6; LCX]

[Homo sapiens tet methylcytosine dioxygenase 1 (TET1), mRNA; Methylcytosine dioxygenase TET1; methylcytosine dioxygenase TET1; CXXC finger 6; tet oncogene 1; CXXC zinc finger 6; ten-eleven translocation-1; CXXC-type zinc finger protein 6; ten-eleven translocation 1 gene protein; leukemia-associated protein with a CXXC domain; tet methylcytosine dioxygenase 1; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Ten-eleven translocation 1 gene protein]

[TET1]

[bA119F7.1; CXXC6; LCX]

[TET1; TET1; LCX; CXXC6; bA119F7.1; CXXC6; KIAA1676; LCX]

TET1_HUMAN

Sf9 insect cells

9601

>90%

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Ships with Dry Ice.

Western Blot (WB)

SDS-PAGE

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant human TET1 (1598-end) was expressed by baculovirus in Sf9 insect cells

Methylcytosine Dioxygenase Proteins; Signaling Proteins - Methylcytosine Dioxygenase Proteins

Recombinant human TET1 (1598-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: TET1 Protein or tet methylcytosine dioxygenase 1 is the original member of a family of methylcytosine dioxygenases that perform several steps required for cytosine demethylation and gene activation (1). TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC. TET1 plays a main role in modulating DNA methylation levels at CpG-rich promoters and also helps in promoting transcription of pluripotency factors as well as participating in the repression of Polycomb-targeted developmental regulators (2).

1. Ito, S. et.al: Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification. Nature 466: 1129-1133, 2010. 2. Wu, H. et.al: Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells. Nature 473: 389-393, 2011.

156139121

NP_085128.2

NM_030625

Q8NFU7

~110 kDa

Function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Ref.1 Ref.7 Ref.8 Ref.9 Ref.11. Catalytic activity: DNA 5-methylcytosine + 2-oxoglutarate + O2 = DNA 5-hydroxymethylcytosine + succinate + CO2. Ref.8 Ref.9DNA 5-hydroxymethylcytosine + 2-oxoglutarate + O2 = DNA 5-formylcytosine + succinate + CO2. Ref.8 Ref.9DNA 5-formylcytosine + 2-oxoglutarate + O2 = DNA 5-carboxylcytosine + succinate + CO2. Ref.8 Ref.9. Cofactor: Binds 1 Fe2+ ion per subunit. Ref.8. Subunit structure: Interacts with HCFC1 and OGT . By similarity. Interacts with SIN3A; recruits the transcriptional corepressor SIN3A to gene promoters. Ref.10. Subcellular location: Nucleus . Probable. Tissue specificity: Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain. Ref.1 Ref.6. Post-translational modification: Glycosylated. Interaction with OGT leads to GlcNAcylation . By similarity. Involvement in disease: A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with KMT2A/MLL1. This is a rare chromosomal translocation 5' KMT2A/MLL1-TET1 3'. Sequence similarities: Belongs to the TET family.Contains 1 CXXC-type zinc finger. Caution: Subsequent steps in cytosine demethylation are subject to discussion. According to a first model cytosine demethylation occurs through deamination of 5hmC into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system (Ref.9). According to another model, cytosine demethylation is rather mediated via conversion of 5hmC into 5fC and 5caC, followed by excision by TDG and replacement by unmethylated cytosine. Sequence caution: The sequence CAD28467.3 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

0.02 mg

270 USD

0.05 mg

450