WWP2 recombinant protein | MyBioSource MBS515118 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

WWP2 recombinant protein

catalog :

MBS515118

quantity :

0.02 mg

price :

260 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS515118

products type :

Recombinant Protein

products full name :

WWP2 recombinant protein

products short name :

WWP2

products name syn :

AIP2; WWp2-like

other names :

Homo sapiens WW domain containing E3 ubiquitin protein ligase 2 (WWP2), transcript variant 1, mRNA; NEDD4-like E3 ubiquitin-protein ligase WWP2; NEDD4-like E3 ubiquitin-protein ligase WWP2; atrophin-1 interacting protein 2; WW domain containing E3 ubiquitin protein ligase 2; Atrophin-1-interacting protein 2; AIP2; WW domain-containing protein 2

products gene name :

WWP2

products gene name syn :

AIP2; WWp2-like

other gene names :

WWP2; WWP2; AIP2; WWp2-like; AIP2

uniprot entry name :

WWP2_HUMAN

host :

Sf9 insect cells

sequence length :

4608

purity :

>90%

form :

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

concentration :

0.1 ug/ul

storage stability :

Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

tested application :

Western Blot (WB)

other info1 :

Accession Number: NM_007014. Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant full-length human WWP2 was expressed by baculovirus in Sf9 insect cells

products categories :

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

products description :

Recombinant full-length human WWP2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: WWP2 or WW domain containing E3 ubiquitin protein ligase 2 is a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. WWP2 contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. WWP2 is an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination (1). WWP2 which is also called AIP2 can bind to atrophin-1 (2).

products references :

1. Xu, H. M. et.al: Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination. J. Biol. Chem. 279: 23495-23503, 2004. 2. Wood, J. D.et.al: Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins. Molec. Cell. Neurosci. 11: 149-160, 1998.

ncbi gi num :

394581978

ncbi acc num :

NP_008945.2

ncbi gb acc num :

NM_007014

uniprot acc num :

O00308

ncbi mol weight :

~130 kDa

ncbi pathways :

Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]

uniprot summary :

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation. Ref.13 Ref.15. Enzyme regulation: Activated by NDFIP1- and NDFIP2-binding. Pathway: Protein modification; protein ubiquitination. Subunit structure: Interacts with POU5F1, RBP1, EGR2 and SLC11A2 . By similarity. Interacts with SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2, ATN1 and adenovirus type 2 PIII. Interacts with ERBB4, NDFIP1 AND NDFIP2. Ref.1 Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.15 Ref.16. Subcellular location: Nucleus Ref.13 Ref.16. Tissue specificity: Detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. Also detected in spleen and peripheral blood leukocytes. Ref.7 Ref.13. Developmental stage: Highly expressed in undifferentiated embryonic stem cells and expression is reduced after embryoid body (EB) formation. Not detectable at day 13 of EB formation; low levels are again detected at day 18 of EB formation. Ref.13. Domain: The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain . By similarity. Ref.12. Post-translational modification: Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome. Ref.14 Ref.18. Miscellaneous: A cysteine residue is required for ubiquitin-thioester formation. Sequence similarities: Contains 1 C2 domain.Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 4 WW domains. Sequence caution: The sequence BAC86528.1 differs from that shown. Reason: Frameshift at position 757.

size1 :

0.02 mg

price1 :

260 USD

size2 :

0.05 mg

price2 :

415

more info or order :

MyBioSource product webpage