protein

CDK8, Unactive recombinant protein

MBS515108

0.02 mg

270 USD

Recombinant Protein

CDK8, Unactive recombinant protein

[CDK8]

[K35; MGC126074; MGC126075; CDK8; Unacitve]

[Homo sapiens cyclin-dependent kinase 8, mRNA; Cyclin-dependent kinase 8; cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8; cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35]

[CDK8]

[K35; MGC126074; MGC126075]

[CDK8; CDK8; K35]

CDK8_HUMAN

Sf9 insect cells

1464

>80%

Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

0.2ug/ul

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: Ships with Dry Ice.

Kinase Assay, Western Blot (WB)

SDS-PAGE

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus. Source Note: Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells

Unactive Kinase; Signaling Proteins - Unactive Kinases

Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets (2).

1.Akoulitchev S, et al: TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000. 2.Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.

47479675

BC069634

P49336

~84 kDa

Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Developmental Biology Pathway (477129); Disease Pathway (530764); FBXW7 Mutants And NOTCH1 In Cancer Pathway (771596); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]

Function: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. Ref.8 Ref.9. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.8ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. Ref.8. Cofactor: Magnesium . By similarity. Subunit structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II. Interacts with CTNNB1, GLI3 and MAML1. Ref.5 Ref.6 Ref.9 Ref.10 Ref.11 Ref.12. Subcellular location: Nucleus . Probable. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.

0.02 mg

270 USD

0.05 mg

450