protein

KDM2A recombinant protein

MBS515102

0.02 mg

270 USD

Recombinant Protein

KDM2A recombinant protein

[KDM2A]

[CXXC8; FBL11; FBL7; FBXL11; JHDM1A; LILINA; KDM2A protein]

[Homo sapiens lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1, mRNA; Lysine-specific demethylase 2A; lysine-specific demethylase 2A; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-rich repeat protein 11; jumonji C domain-containing histone demethylase 1A; jmjC domain-containing histone demethylation protein 1A; lysine (K)-specific demethylase 2A; CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A]

[KDM2A]

[CXXC8; FBL11; FBL7; FBXL11; JHDM1A; LILINA]

[KDM2A; KDM2A; FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA; CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004]

KDM2A_HUMAN

Sf9 insect cells

[1-748]

7393

>90%

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

0.1 mg/ml

Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Shipping: Ships with dry ice. Stability: 1 year at -70°C from date of shipment.

Western Blot (WB)

Testing Data

Type: Recombinant Fusion Protein. Species: Human. Tag Information: GST tag. Expression System: Sf9 insect cells using baculovirus

Deacetylases/Demethylase Proteins; Signaling Proteins - Deacetylase/Demethylase Proteins

Recombinant human KDM2A (1-748) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Scientific Background: KDM2A or lysine (K)-specific demethylase 2A is a member of the F-box protein family that are components of the modular E3 ubiquitin protein ligases called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination (1). KDM2A belongs to the Fbls class and, in addition to the presence of an F-box, contains at least six highly degenerated leucine-rich repeats which play a role in epigenetic silencing. The demethylase activity of the JmjC domain-containing proteins is conserved from yeast to human (2).

1. Cenciarelli, C.et.al: Identification of a family of human F-box proteins. Curr. Biol. 9: 1177-1179, 1999. 2. Tsukada, Y. et.al: Histone demethylation by a family of JmjC domain-containing proteins. Nature 439: 811-816, 2006.

226437603

NP_036440.1

NM_012308

Q9Y2K7

115kDa

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Function: Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Ref.12 Ref.14. Catalytic activity: Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2. Ref.12Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2. Ref.12. Cofactor: Binds 1 Fe2+ ion per subunit. Ref.12. Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex . By similarity. Interacts with CBX5/HP1A; the interaction promotes CBX5 localization to chromatin. Ref.14. Subcellular location: Nucleus nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. Ref.14 Ref.19. Tissue specificity: Widely expressed, with highest levels in brain, testis and ovary, followed by lung. Ref.4. Domain: The JmjC domain mediates demethylation activity and is required for satellite silencing. Ref.12 Ref.19The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated. Ref.12 Ref.19. Sequence similarities: Belongs to the JHDM1 histone demethylase family.Contains 1 CXXC-type zinc finger.Contains 1 F-box domain.Contains 1 JmjC domain.Contains 6 LRR (leucine-rich) repeats.Contains 1 PHD-type zinc finger. Sequence caution: The sequence AAD56012.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA76848.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB15795.1 differs from that shown. Reason: Frameshift at position 410. The sequence BAJ05817.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

0.02 mg

270 USD

0.05 mg

450