Sheep anti-rat von Willebrand Factor, Whole IgG from antiserum | MyBioSource MBS512181 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Sheep anti-rat von Willebrand Factor, Whole IgG from antiserum

catalog :

MBS512181

quantity :

5 mg

price :

310 USD

clonality :

polyclonal

host :

sheep

conjugate :

nonconjugated

reactivity :

dog

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS512181

products type :

Antibody

products full name :

Sheep anti-rat von Willebrand Factor, Whole IgG from antiserum

products short name :

[von Willebrand Factor]

products name syn :

[von Willebrand Factor, rat]

other names :

[von Willebrand factor; von Willebrand factor; von Willebrand factor]

other gene names :

[VWF; VWF; F8VWF; F8VWF]

uniprot entry name :

VWF_CANFA

clonality :

Polyclonal

host :

Sheep

specificity :

This antibody is specific for rat vWF as demonstrated by immunoelectrophoresis and ELISA.

purity :

Purified IgG.

form :

Whole IgG, clear liquid.

concentration :

IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4.

storage stability :

Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

app notes :

Suitable for use as a source of antibodies to rat vWF.

other info1 :

Note: Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 5 mg. Immunogen: Rat vWF purified from plasma.

other info2 :

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol. Neutralizing Activity: Not determined

products description :

von Willebrand Factor (vWF, also previously referred to as Factor VIII related antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF, the first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib, the second being the binding and stabilization of Factor VIII (antihemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95,000 dalton propeptide (also known as vWF antigen-II), believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies) after which the propeptide is cleaved and released. vWF circulates as multimers of disulphide linked 220,000 dalton subunits and the molecular weight of these multimers ranges from 0.5- 20 million daltons. The concentration of vWF in human plasma is typically 10 ug/ml, but increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand's disease (vWD) is perhaps the most common inherited bleeding disorder in humans and is the result of either quantitative deficiencies of vWF (vWD Types I & III), or one of a number of qualitative disorders of vWF structure and function (vWD Type II).

products references :

1. Montgomery RR, Coller BS, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 134-168, J.B. Lippincott Co., Philadelphia PA, USA, 1994. 2. Sadler JE; von Willebrand Factor (Minireview); JBC 266:34, pp 22777- 22780, 1991.

ncbi gi num :

50950127

ncbi acc num :

NP_001002932.1

ncbi gb acc num :

NM_001002932.1

uniprot acc num :

Q28295

ncbi mol weight :

309533 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198608); Complement And Coagulation Cascades Pathway (84123); Complement And Coagulation Cascades Pathway (484); ECM-receptor Interaction Pathway (84118); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (84117); Focal Adhesion Pathway (478); Formation Of Platelet Plug Pathway (363620); GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway (363617); Hemostasis Pathway (363619)

uniprot summary :

Function: Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Subunit structure: Multimeric. Interacts with F8. Ref.2. Subcellular location: Secreted. Secreted extracellular space extracellular matrix. Note: Localized to storage granules. Ref.2. Tissue specificity: Plasma. Domain: The propeptide is required for multimerization of vWF and for its targeting to storage granules. Post-translational modification: All cysteine residues are involved in intrachain or interchain disulfide bonds. By similarity.N- and O-glycosylated. Involvement in disease: Note=Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF. Ref.4. Sequence similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 4 TIL (trypsin inhibitory-like) domains.Contains 3 VWFA domains.Contains 3 VWFC domains.Contains 4 VWFD domains.

size1 :

5 mg

price1 :

310 USD

more info or order :

MyBioSource product webpage